The Production of Ginsenosides from Ginseng Hairy Root by Treatment of the Chitin and Chitosan

Chitin과 Chitosan 처리에 의한 인삼모상근으로부터 Ginsenosides 생산

To elucidate the effect of chitin and chitosan on the production of ginsenosides and growth, ginseng hairy root was cultured on the 1/2 MS medium supplemented with chitin and chitosan of various concentrations and culture period. The highest growth was obtained with 1 mg/L of chitin. However, the growth was inhibited by 20 mg/L or above. The contents and productivity of ginsenosides were the highest when ginseng hairy roots were cultured on 40 mg/L chitin and applied of the third-weeks of culture period. Ginseng hairy root culture with 1 mg/L of chitosan resulted in the best growth, but the highest ginsenosides level was appeared in 30 mg/L chitosan. Ginsenosides content was increased when it was treated at the forth-week after culture as 30 mg/L of chitosan.

인삼모상근의 생장과 glnsenosides 함량 증가에 미치는 chitin 및 chitosan의 효과를 규명하기 위해, 식물생장조절제가 첨가되지 않은 1/2 MS 배지에 chitin과 chitosan의 농도와 처리시기를 달리하여 인삼모상근을 배양하였다. 인삼모상근 생장은 chitin 1 mg/L의 농도에서 가장 양호하였으나 20mg/L 이상 농도에서는 생장이 감소되었다. Ginsenosldes 함량과 생산성은 40 mg/L 첨가시와 배양3주 째 처리구에서 가장 높았다. Chitosan 역시 1 mg/L의 농도에서 인삼모상근의 생장이 가장 높았으나 ginsenosides 함량은 30 mg/L 처리구에서 가장 높게 나타났고 배양 후 처리시기는 ginsenosides의 함량증가에 큰 영향을 미치지 않았다.