Zinc-induced Apoptosis in C6 glial Cells via Generation of Hydrogen Peroxide($H_2O_2$)

신경교세포주 C6 glial에서 Zinc의 Hydrogen Peroxide($H_2O_2$) 생성을 통한 세포고사

  • 이지현 (원광대학교 의과대학 미생물학교실, 한의학전문대학원) ;
  • 김명선 (원광대학교 의과대학 미생물학교실) ;
  • 소흥섭 (원광대학교 의과대학 미생물학교실) ;
  • 김남송 (원광대학교 의과대학 예방의학교실) ;
  • 조광호 (원광대학교 의과대학 한의학전문대학원) ;
  • 이향주 (원광대학교 의과대학 미생물학교실) ;
  • 이기남 (원광대학교 의과대학 한의학전문대학원) ;
  • 박길래 (원광대학교 의과대학 미생물학교실, 한의학전문대학원)
  • Published : 2000.09.01

Abstract

Zinc is known to generate reactive oxygen species (ROS) including superoxide anion and hydrogen peroxide ($H_2O_2$), which eventually contribute to cytotoxicity in a variety of cell types. Here in, we demonstrated that zinc decreased the viability of C6 glial cells in a time and dose-dependent manner, which was revealed as apoptosis characterized by ladder-pattern fragmentation of genomic DNA. chromatin condensation and DNA fragmentation in Hoechst dye staining. Zinc-induced apoptosis of C6 glial cells was prevented by the addition of catalase and antioxidants including reduced glutathione (GSH), N-acetyl-L-cysteine (NAC) and pyrrolidinedithiocarbamate (PDTC). Wefurther confirmed that zinc decreased intrac-ellular levels of GSH and generated $H_2O_2$in C6 glial cells. Moreover, antioxidants also decreased the generation of zinc-induced $H_2O_2$ in C6 glial cells. These data indicated that zinc-induced the apoptotic death of C6 glial cells via generation of reactive oxygen species such as $H_2O_2$.