Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
권호 표지

Degradation of Microcystins during the Decomposition Process of Cyanobacterial Cells

Cyanobacteria의 분해에 따른 Microcystins의 변화

  • Shin, Jae-Ki (Environmental Research Team, Water Resources Research Institute, Korea Water Resources Corporation) ;
  • Yim, Seong-A (Environmental Research Team, Water Resources Research Institute, Korea Water Resources Corporation) ;
  • Choi, Il-Hwan (Environmental Research Team, Water Resources Research Institute, Korea Water Resources Corporation)
  • 신재기 (한국수자원공사 수자원연구소) ;
  • 임성아 (한국수자원공사 수자원연구소) ;
  • 최일환 (한국수자원공사 수자원연구소)
  • Published : 2000.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The decomposition processes of Microcystis aeruginosa under the light and dark conditions were investigated in relation to the change of microcystins, physicochemical and biological factors. Cyanobacterial cells from upper stream of Lake Dae-chong were collected and incubated in the matrix of raw water under the light and dark conditions without additional nutrients. The decomposition of Microcystis cells started from beginning of the experiment and most of the cells were decomposed on 12th day. Under the light condition the concentration of toxins in filtrate fractionwas increased with the increase of viscosity as the decomposition of algal cells proceed whereas no significant change was observed under the dark condition. Microcystin- RR was most labile toxin than the other two microcystins because it was identified mainly in lyophilized cells but detected at trace level in the filtratefraction.

본 연구에서는 남조류의 독성물질 배출 및 분해양상을 밝히고자 대청댐 지류인 옥천천의 스컴을 채취, 실험대상으로 하였다. Light와 dark 상태로 분리${\cdot}$배양한 배지에는 조사를 하는 동안 다른 영양염류는 첨가하지 않았다. 그 결과 세포내에서 microcystins의 총량은 세포수가 감소함에 따라 감소하였으며 Microcystis aeruginosa의 현존량과 비교하였을 때 light와 dark 상태의 경우 $r^{2}$값이 각각 0.96과 0.97로 높은 상관관계를 보였고 dark 상태에서 보다 더 빠른 감소추세를 보였다. 또한 세포에서 수체로 용존되는 microcystins의 량은 세포내에 존재하는 양의 극히 일부임을 확인 할 수 있었고 세포내에 분포하는 microcystins의 농도는 종류에 따라 차이를 보여 microcystin-RR>-LR>-YR의 순으로 존재하는 것으로 나타났다. 이러한 결과는 옥정호과 제천천을 대상으로 한 수심별 조사에서도 동일한 양상을 보였다. 수체에서 검출된 microcystins의 양은 점성물질의 양과 함께 light와 dark 상태에 따라 뚜렷한 차이를 보였으며 light의 경우 $r^{2}$값이 -0.75의 역의 상관관계를 보였다. 남조류 세포 및 microcystins에 대한 light와 dark 상태에서의 서로 다른 농도 결과는 배양 상태에 따른 미생물의 분해양상의 차이와 미생물에 대한 점성물질의 영향으로 추정된다.

Keywords

References

  1. 국내 호수에서 발생하는 남조류 독소의 분포, 한국수질보전학회 춘계학술발표회 논문초록집 김범철;김호섭;유민철;최연규;박호동;최일환
  2. 한국산 남조류 Microcystis spp.의 생리.생태적 연구 박혜경
  3. Korean J. Limnol. v.31 no.4 Composition of Microcystin from Cyanobacterial Water Blooms of the Sonaktong Reservoir, Korea 이진애;Vishal C. Srivastava;최애란;김우현;박미정
  4. 낙동강 수계에서의 남조류 독성 연구 및 그 제거 방안. 국립환경연구원 연구용역보고서 이진애;조경제;권재현;Srivastava, V.;이채언;손혜숙
  5. The Report of Water Quality Research Institute of Pusan v.4 남조류 (cyanobacteria)의 microcystins 분석과 동태 정종문;최은재;박홍기;박은주;정철원;김주헌
  6. '99 서울 상수도 기술세미나 남조류발생 유해성 독소에 관한 조사.연구 조석주;이수원;조은주;김재돈;최종갑
  7. J. of Korean Ind. & Eng. Chemistry v.8 한국산 남조류 Microcystis로부터 생산된 microcystin 구조와 생물 활성에 관한 연구 최병욱;노영호;이종수
  8. Jpn. J. Limnol. v.52 Regeneration of nutrient and detritus formation from aerobic decomposition of natural phytoplankton Aizaki, M.;Takamura, N.
  9. J. Chem. Soc. Perkin Trans. v.1 The structure of cyanoginosin-LA, a cyclic heptapeptide toxin from the cyanobacterium Microcystis aeruginosa Botes, D.P.;Tuiman, A.A.;Wessels, P.L.;Viljoen, C.C.;Kruger, H.;Williams, D.H.;Santikarn, S.;Smith, R.J.;Hammond, S.J.
  10. J. Chem. Soc. Perkin Trans. v.1 Structural studies on cyanoginocin-LR, - YR, -YA and -YM peptide toxins from Microcystis aeruginosa Botes, D.P.;Wessels, P.L.;Kruger, H.;Runnegar, M.T.C.;Santikarn, S.;Smith, R.J.;Barna, J.C.J.;Williams, D.H.
  11. Appl. envir. Microbiol. v.62 Enzymatic pathway for the bacterial degradation of the cyanobacterial cyclic peptide toxin microcystin-LR Bourne, D.G.;Jones, G.J.;Blakeley, R.L.;Jones, A.;Negri, A.P.;Riddles, P.
  12. Toxicon v.26 Naming of cyclic heptapeptide toxins of cyanobacteria (blue-green algae) Carmichel, W.W.;Beasly, V.;Bunner, D.L.;Eloff, J.N.;Falconer, I.;Gorham, P.;Harada, K.I.;Krishnamurthy, T.;Min-Juan, Y.;Moore, R.E.;Rinehart, K.;Runnegar, M.;Skulberg, O.M.;Watanabe, M.F.
  13. The occurrence and fate of blue-green algal toxins in freshwaters. London, Her Majesty’s Stationery Office (National Rivers Authority R and D Report 29) Codd, G.A.;Bell, S.G.
  14. Water research v.30 Biodegradation of microcystin-LR by indigenous mixed bacterial populations Cousins, I.T.
  15. Structure/Toxicity Relationships and Fate of Low Molecular Weight Peptide Toxins from Cyanobacteria Dahlem, A.M.
  16. Phycologia v.35 Tumor promotion by cyanobacterial toxins Falconer, I.R.;Humpage, A.R.
  17. The Medical Journal of Austrlia v.1 Evidence of liver damage by toxin from a bloom of the blue-green alga, Microcystis aeruginosa Falconer, I.R.;Beresford, A.M.;Runnegar, M.T.C.
  18. Wat. Res. v.32 no.10 Optimised Extraction of Microcystins from Field Samples-A Comparison of Different Solvents and Procedures Fastner, J.;Flieger, I.;Neumann, U.
  19. J. Toxicol. Toxin reviews v.17 no.3 Persistent and Decomposition of Heptatotoxic Microcystins Produced by Cyanobacteria in Natural Environment Harada, K.I.;Kiyomi, Tsuji
  20. J. Chromatogr v.448 Harada, K.I.;Oishi, S.;Dahlem, A.M.;Beasley, V.R.;Carmichael, W.W.
  21. Environ. Toxicol. Water Qual. v.12 Nodularin concentrations in Lakes Alexandria and Albert, South Austria, during a bloom of the cyanobacterium Nodularia spumigena and degradation of the toxin Heresztyn, T.;Nicholson, B.C.
  22. Health risk assessment of microcystins in drinking water supplies. Toxic cyanibacteria-a global perspective Hurdey, S.E.;Lambert, T.W.;Kenefick, S.L.
  23. The Analysis of Microcystin-LR in Water, Application in Water Treatment Studies. Detection Methods for Cyanobacterial Toxins James, H.A.;James, C.P.;Hart, J.
  24. Water research v.28 Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay Jones, G.J.;Orr, P.T.
  25. Polymer rheology Nielsen, L.E.
  26. Arch. Hydrobiol. Beih. v.14 Comparison of different methods for chlorophyll and phaeopigment determination Nusch, E.A.
  27. Water Quality Series v.2 Toxic blue-green algae. A report by the UK National Rivers Authority Pearson, M.J.
  28. Letter in Applied Microbiology v.19 Biodegradability and adsorption on sediments of cyanobacterial hepatotoxins and anatoxins-a Ralpala, J.;Lahti, K.;Sivonen, K.;Niemela, S.I.
  29. Health effects of toxic cyanobacteria (blue-green algae.) Ressom, R.
  30. Laboratory manual for the examination of water, wastewater and Soil Rump, H.H.;Krist, H.
  31. Jpn. J. Limnol. v.54 Toxic water bloom of blue-green algae. Biological and chemical characteristics Watanabe, M.F.;Harada, K.I.
  32. Bulletin of the Japanese Society of Scientific Fisheries v.49 A highly toxic strain of blue green alga Microcystis aeruginosa isolated from Lake Suwa Watanabe, M.F.;Oishi, S.
  33. Proceedings of an International Workshop Blue-green algae and river cancer. Toxic cyanobacteria, current status of research and management Yu, S.Z.;Steffensen, D.A.(ed.);Nicholson, B.C.(ed.)