Identification and antimicrobial susceptibility of bacteria from the uterus of bitches with pyometra

개 자궁축농증에서의 원인균 분리 및 항생제 감수성 검사

  • Lee, So-hyun (Lab of Theriogenology, Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Jong-ki (Lab of Theriogenology, Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University) ;
  • Shin, Na-ri (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Hye-soo (Lab of Theriogenology, Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University) ;
  • Yong, Hwan-yul (Lab of Theriogenology, Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University) ;
  • Yoo, Han-sang (Lab of Theriogenology, Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Kang-nam (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Byeong-chun (Lab of Theriogenology, Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University) ;
  • Hwang, Woo-suk (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
  • Accepted : 2000.11.25
  • Published : 2000.12.20

Abstract

This study was performed to isolate and identify bacteria from uterus with pyometra and examine their susceptibility to antimicrobial agents. Uterus of 16 bitches with pyometra were surgically removed by ovariohysteroctomy and then bacteria were isolated and identified. Also, susceptibility test to 15 antimicrobial agents was performed. Out of 16 bitches, 11 strains of Escherichia coli, 2 strains of Serratia marcescens, and 1 strain of Staphylococcus aureus and Salmonella spp. were identified. In antimicrobial susceptibility test, the majority of isolates were susceptible to enrofloxacin, norfloxacin, chloramphenicol, nalidixic acid, gentamicin, trimethprim-sulfamethazole, tetracycline, and moderately susceptible to carbenicillin, amikacin, ampicillin, neomycin, but resistant to vancomycin, streptomycin, bacitracin and colistin. In conclusion, E coli was the most common bacteria isolated from bitches with pyometra and those susceptible antimicrobial agents could be recommended to medical therapy of pyometra.

본 연구는 1998년부터 1999년까지 서울대학교 수의과대학 부속동물병원에 내원한 개중 자 궁축농중으로 진단된 개의 자궁내용물로부터 균을 분리, 동정하고 항생제 감수성 검사를 실시하여 개 자궁축농증 치료를 위한 기초 자료로 사용하고자 하였다. 위 병원에 내원하여 자궁축농증으로 진단된 개는 16두였으며, 이들로부터 자궁적출술을 이용하여 자궁을 적출한 후 원인균을 분리하였고, 분리된 균에 대하여 15종의 항균제에 대하여 감수성 검사를 실시하였다. 16두의 개로부터 분리된 균은 Escherichia coli 11주, Serratia marcescens 2주, Staphylococcus aureus 및 Salmonella spp 각 l주 씩 이었다. 이들 균주들에 대하여 항균제 감수성 검사를 실시한 결과, 대부분의 균주들이 enrofloxacin, norfloxacin, chloramphenicol, nalidixic acid, gentamicin, Trimethprim-sulfamethazole, tetracycline에 대하여는 높은 감수성을 나타내었고, carbenicillin, amikacin, ampicillin, neomycin에 대하여는 중등도의 감수성을 나타내었으나, vancomycin, streptomycin, bacitracin, colistin에 대하여는 저항성을 나타내었다.

Keywords