Korean Journal of Veterinary Research (대한수의학회지)
- Volume 40 Issue 1
- /
- Pages.81-85
- /
- 2000
- /
- 2466-1384(pISSN)
- /
- 2466-1392(eISSN)
Development of competitive enzyme linked immunosorbent assay for detection of Coxiella burnetii antibody in animal
동물에서 Coxiella burnetii 항체를 진단하기 위한 경쟁효소면역법 개발
-
Cho, Dong-hee
(National Veterinary Research and Quarantine Service)
;
- Kim, Yong-ju (National Veterinary Research and Quarantine Service) ;
-
Wee, Sung-hwan
(National Veterinary Research and Quarantine Service)
;
- Cho, Mi-young (National Veterinary Research and Quarantine Service) ;
-
Kweon, Chang-hee
(National Veterinary Research and Quarantine Service)
;
- Kang, Yung-bai (National Veterinary Research and Quarantine Service) ;
-
Park, Yong-ho
(College of Veterinary Medicine, Seoul National University)
;
-
Cho, Sang-nae
(College of Medicine, Yonsei National University)
-
조동희
(국립수의과학검역원)
;
- 김용주 (국립수의과학검역원) ;
-
위성환
(국립수의과학검역원)
;
- 조미영 (국립수의과학검역원) ;
-
권창희
(국립수의과학검역원)
;
- 강영배 (국립수의과학검역원) ;
-
박용호
(서울대학교 수의과대학)
;
-
조상래
(연세대학교 의과대학)
- Received : 2000.01.04
- Published : 2000.03.25
Abstract
Coxiella burnetii (C burnetii) is the causative agent of Q fever in animal and human. The distribution of the disease has been documented around world. In this study we developed the competitive enzyme linked immunosorbent assay(cELISA) and compared it with indirect immunofluorescent assay(IFA). A monoclonal antibody(Mab) against C burnetii and a peroxidase-conjugated anti-mouse IgM were used as an indicator system competing against antibody in animal serum or as an indicater of the absence of antibody. Sera were considered antibody positive when the percentage inhibition index(PI index) is upper than 30. PI index is calculated as 100-[sample OD/Mab OD)