Korean Journal of Veterinary Research (대한수의학회지)
- Volume 40 Issue 3
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- Pages.543-549
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- 2000
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Studies on the safety of Brucella abortus RB51 vaccine II. Safety of Brucella abortus RB51 in mouse and guinea pig
부루세라백신(BR51)의 안전성에 관한 연구 II. 부루세라 RB51 백신균주의 마우스 및 기니픽에 대한 안전성
- Kim, Jong-man (National Veterinary Research and Quarantine Service) ;
- Woo, Sung-ryong (National Veterinary Research and Quarantine Service) ;
- Lee, Ji-youn (National Veterinary Research and Quarantine Service) ;
- Kim, Sun-young (National Veterinary Research and Quarantine Service) ;
- Kweon, Chang-hee (National Veterinary Research and Quarantine Service) ;
- Jean, Young-hwa (National Veterinary Research and Quarantine Service) ;
- Yoon, Yong-dhuk (National Veterinary Research and Quarantine Service) ;
- Cho, Sang-nae (College of Medicine, Yonsei University)
- 김종만 (국립수의과학검역원) ;
- 우승룡 (국립수의과학검역원) ;
- 이지연 (국립수의과학검역원) ;
- 김선영 (국립수의과학검역원) ;
- 권창희 (국립수의과학검역원) ;
- 진영화 (국립수의과학검역원) ;
- 윤용덕 (국립수의과학검역원) ;
- 조상래 (연세대학교 의과대학)
- Received : 2000.08.16
- Published : 2000.09.25
Abstract
The pathogenicity of Br abortus RB51 strains, producted by commercial vaccine companies in Republic of Korea and USA, were evaluated in mouse and guinea pig. BALB/c and ICR mice were intraperitoneally inoculated with RB51 vaccines or virulent field strain and the existence of RB51 including its ratio of spleen to weights and persistence in spleens were examined. Groups of guinea pigs on day 55-58 of received subcutaneously with various RB51 vaccines, RB51 field isolates (Daehungjin) or virulent field isolates(Sangju) to compare the histopathogenicity in uterus. All the mice received RB51 vaccines or RB51 field isolates survived for 10 days, but the groups of mice received virulent field isolates died 5 from 11 (45.5%) in case of BALB/c mice and 12 from 12 (100%) in ICR mice, respectively. The number of RB51 in the groups of mice given with vaccine strains and RB51 field isolates were declined rapidly were in spleens between 12 and 20 days after inoculation. In contrast the mice given with the virulent field isolates rose in number of bacteria up to 20 days after inoculation. In the groups of mice infected with virulent field isolates, the ratio of spleen weights to body weight were significantly higher than those in control or in the groups inoculated with RB51 strain, including RB51 field isolates, at 12 and 20 days after inoculation. At ten days after inoculation, placentas of both the pregnant and non-pregnant guinea pigs were conducted for histopathological examination. Although any abnormal lesions were not observed in non-pregnant guinea pigs, all the strains caused the inflammation of the placenta, implying pathogenecity of RB51 in pregnant guinea pigs.
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