한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제33권2호
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- Pages.103-109
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- 2000
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
미토콘드리아 ribosomal RNA 유전자 염기서열분석에 의한 한국산 연어아과 어류의 유전적 계통도
Phylogeny of the subfamily Salmoninae distributed in Korea based upon nucleotide sequences of mitochondrial ribosomal RNA genes
- 이희정 (국립수산진흥원 생물공학과) ;
- 박중연 (국립수산진흥원 생물공학과) ;
- 이정호 (국립수산진흥원 생물공학과) ;
- 민광식 (국립수산진흥원 생물공학과) ;
- 전임기 (국립수산진흥원 증식부) ;
- 유미애 (부산대학교 분자생물학과) ;
- 이원호 (부산대학교 생물학과)
- LEE Heui-Jung (Biotechnology Division, National Fisheries Research & Development Institute) ;
- PARK Jung-Youn (Biotechnology Division, National Fisheries Research & Development Institute) ;
- LEE Jeong-Ho (Biotechnology Division, National Fisheries Research & Development Institute) ;
- MIN Kwang-Sik (Biotechnology Division, National Fisheries Research & Development Institute) ;
- JEON Im Gi (Aquaculture Division, National Fisheries Research & Development Institute) ;
- YOO Mi-Ae (Department of Molecular Biology, Pusan National University) ;
- LEE Won-Ho (Department of Biology, Pusan National University)
- 발행 : 2000.03.01
초록
열목어를 비롯한 산천어, 시마연어, 연어, 무지개송어 등 우리나라 연어아과 어류의 집단구조분석을 위한 기초자료를 얻기 위하여, 미토콘드리아 ribosomal RMA 유전자 영역의 염기서열변이를 비교
Complete senuences of the mitochondrial rRNA Benes were determined among six salmonines in Korean Waters (Brachpmystax lenok, Onoorhpchus keta, O. masou mason, O. mason ishikawae, O. mykiss, and albino mutant of O. mykiss). The purposes of this study were to provide the basic information on levels of mtDNA polymorphism among these species for genetic characterization; discuss phylogentic relationships among three Oncorhynchus sepecies; demonstrate the utility of rRNA gene sequence data as a genetic marker for disringuishinf among Korean salmonines. PCR/direct sequencing data indicated the following consistent results; 1) 12S rRNA genes was 945 bases long in Oncorhynchus species, and 946 bases in B. lenot including one insertion. 2) Of sequence variation in mitochondrial rRNA regions, transitional substitutions were superior to transversion. 3) The significant differences were not shown in the intraspecific variation values in these gene regions. The percentage sequence divergence values were ranged from