Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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E1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine

E1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구

  • Yoon, Soo Han (Department of Neurosurgery, Ajou University School of Medicine) ;
  • Kim, Jin Young (Department of Neurosurgery, Ajou University School of Medicine) ;
  • Park, Seung Woo (Department of Neurosurgery, Ajou University School of Medicine) ;
  • Ahn, Young Hwan (Department of Neurosurgery, Ajou University School of Medicine) ;
  • Ahn, Young Min (Department of Neurosurgery, Ajou University School of Medicine) ;
  • Cho, Ki Hong (Department of Neurosurgery, Ajou University School of Medicine) ;
  • Cho, Kyung Gi (Department of Neurosurgery, Ajou University School of Medicine)
  • 윤수한 (아주대학교 의과대학 신경외과학교실) ;
  • 김진영 (아주대학교 의과대학 신경외과학교실) ;
  • 박승우 (아주대학교 의과대학 신경외과학교실) ;
  • 안영환 (아주대학교 의과대학 신경외과학교실) ;
  • 안영민 (아주대학교 의과대학 신경외과학교실) ;
  • 조기홍 (아주대학교 의과대학 신경외과학교실) ;
  • 조경기 (아주대학교 의과대학 신경외과학교실)
  • Received : 1999.09.03
  • Accepted : 1999.11.16
  • Published : 2000.06.28
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Abstract

Purpose : The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immunohistochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein. Material and Method : We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope. Results : E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine. Conclusion : These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.

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