Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellular Matrix

Extracellular Matrix 배지에서 생쥐 배아의 발생 및 아폽토시스

  • Kang, Byung-Moon (Department of Obsterics and Gynecology, College of Medicine, Ulsan University) ;
  • Sohn, In-Pyo (Department of Biology, Natural Sciences, Kyonggi University) ;
  • Chung, Byung-Mok (Department of Biology, Natural Sciences, Kyonggi University) ;
  • Choi, Kyoo-Wan (Biomed Institute) ;
  • Gye, Myung-Chan (Department of Biology, Natural Sciences, Kyonggi University)
  • 강병문 (울산대학교 의과대학 산부인과) ;
  • 손인표 (경기대학교 자연과학부 생물학과) ;
  • 정병목 (경기대학교 자연과학부 생물학과) ;
  • 최규완 (바이오메드 연구소) ;
  • 계명찬 (경기대학교 자연과학부 생물학과)
  • Published : 2000.09.30
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Abstract

Objective: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm (EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. Method: Late pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assesed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. Results: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. Conclusion: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.

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