Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지

Vitrification and Ultrarapid Freezing of Day 2 Mouse Embryos

제 2일째 생쥐 배아의 초자화동결과 초급속동결

  • Yang, Jung-Sook (Taehak Infertility Clinic) ;
  • Sohn, Cherl (Taehak Infertility Clinic) ;
  • Bae, In-Ha (Department of Biology, College of Natural Sciences, Sungshin Women's University)
  • 양정숙 (대학 산부인과) ;
  • 손철 (대학 산부인과) ;
  • 배인하 (성신여자대학교 자연과학대학 생물학과)
  • Published : 2000.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. Methods: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. Results: We tested toxicity by exposing embryos to vitirification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9% (EFS), 48.5% (VS14) and 70.1% (UFS). Conclusion: The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.

Keywords