BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Characterization of KI-24, a Novel Murine Monoclonal Antibody with Specific Reactivity for the Human Immunodeficiency Virus-1 p24 Protein

  • Shin, Song-Yub (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Jung-Hyun (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Myung-Kyu (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jang, So-Youn (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hahm, Kyung-Soo (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 1999.08.28
  • Accepted : 1999.10.07
  • Published : 2000.01.31
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Abstract

The HIV-1 p24(202-221) sequence ETINNEEEWDRVHPV HAGP contains a B-cell epitope with the earliest immune response and the highest antibody titer against anti-mouse sera obtained by immunization with p24 antigens. A novel mouse monoclonal antibody (mAb) was generated against the immunodominant B-cell epitope of the HIV-1 p24 capsid protein, p24(202-221). BALB/c mice were immunized with the four branched multiple antigenic peptide (MAP) containing the HIV-1p24(202-221) sequence, and antibody-secreting hybridoma were produced by fusion of mouse splenocytes with P3X63Ag8.653, mouse myeloma cells. One clone which produced the antigen-specific mAb named KI-24 (Isotype IgG1, light chain: ${\kappa}$) was identified. mAb KI-24 was highly specific for both the p24(202-221) and p24 proteins when analyzed by ELISA and Western blotting. Since p24(202-221) also contains a cytotoxic T-lymphocyte epitope, this specfic peptide epitope and the monoclonal antibody with specific reactivity against the p24 protein and p24(202-221) can be used in peptide vaccine development and p24 antigen detection from HIV patients.

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