High Level Production of Glycoprotein H of HSV-1 (F) Using HcNPV Vector System

  • Kang, Hyun (Department of Biological Science & Institute for Genetic Engineering, Konkuk University) ;
  • Cha, Soung-Chul (Department of Biological Science & Institute for Genetic Engineering, Konkuk University) ;
  • Han, You-Jin (Department of Biological Science & Institute for Genetic Engineering, Konkuk University) ;
  • Park, In-Ho (Department of Biological Science & Institute for Genetic Engineering, Konkuk University) ;
  • Lee, Min-Jung (Department of Biological Science & Institute for Genetic Engineering, Konkuk University) ;
  • Byun, Si-Myung (Department of Biological Science, Korea Advanced Institute of Science and Technology) ;
  • Lee, Hyung-Hoan (Department of Biological Science & Institute for Genetic Engineering, Konkuk University)
  • Received : 2000.10.18
  • Accepted : 2000.11.09
  • Published : 2000.11.30

Abstract

The Herpes simplex virus type 1 (HSV-1) strain F glycoprotein H (gH) gene in the pHLB-4 plasmid was recombinated into a baculovirus expression vector (lacZ-HcNPV) to construct a recombinant virus GH-HcNPV expressing gH. The sequences of gH and its expression were analyzed. The gH gene was located in the 6.41 kb BglII fragment. The open reading frame (ORF) of the gH gene was 2,517 bp and codes 838 amino acid residues. Insect cells infected with this recombinant virus synthesized a high level of the matured and gX-gH fusion protein with approximately 112 kDa. The fusion gH protein was localized on the membrane of the insect cells as seen by using immunofluorescence assay and accumulated in the cultured media by the SDS-PAGE and immunoprecipitation assays. The amino acid sequence presents additional characteristics compatible with the structure of a viral glycoprotein: signal peptide, putative glycosylation sites and a long C-terminal transmembrane sequence. Antibodies raised in mice to this recombinant protein recognized viral gH and neutralized the infectivity of HSV-1 in vitro. These results demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV; accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

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