대한약침학회지 (Journal of Pharmacopuncture)

  • 제4권1호
  • /
  • Pages.65-84
  • /
  • 2001
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
DOI QR코드

DOI QR Code

The antinociceptive and anti-inflammatory effect of water-soluble fraction of bee venom on rheumatoid arthritis in rats

  • Lee, Jang-Hern (Department of Veterinary Physiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kwon, Young-Bae (Department of Veterinary Physiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Jae-Dong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Kang, Sung-Keel (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University) ;
  • Lee, Hye-Jung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University)
  • 발행 : 2001.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

We recently demonstrated that bee venom (BV) injection into acupoint (i.e. Zusanli) produced more potent anti-inflammatory and antinociciptive effect in Freunds adjuvant induced rheumatoid arthritis (RA) model as compared with that of non-acupoint injection(i.e back). However, the precise components underlying BV-induced antinociceptive and/or anti-inflammatory effects have not been fully understood. Therefore, we further investigated the anti-arthritic effect of BV after extracting the whole BV according to solubility (water soluble: BVA, ethylacetate soluble: BVE). Subcutaneous BVA treatment (0.9 mg/kg/day) into Zusanli acupoint was found to dramatically inhibit paw edema and radiological change (i.e. new bone proliferation and soft tissue swelling) caused by Freunds adjuvant injection. In addition, the increase of serum interleukin-6 by RA induction was normalized by the BVA treatment as similar with that of non-arthritic animals. On the other hand, BVA therapy significantly reduced arthritis induced nociceptive behaviors (i.e., nociceptive score for mechanical hyperalgesia and thermal hyperalgesia). Furthermore, BVA treatment significantly suppressed adjuvant induced Fos expression in the lumbar spinal cord at 3 weeks post-adjuvant injection. However, BVE treatment (0.05 mg/kg/day) has not any anti-inflammatory and anti-nociceptive effect on RA. Based on the present results, we demonstrated that BVA might be a effective fraction in whole BV for long-term treatment of RA-induced pain and inflammation. However, it is clear necessary that further fraction study about BVA was required for elucidating an effective component of BVA.

키워드

참고문헌

  1. Neuroscience v.48 c-fos expression in rat lumbar spinal cord during the development of adjuvant-induced arthritis Abbadie, C.;Besson, J.M. https://doi.org/10.1016/0306-4522(92)90287-C
  2. Pain v.57 Chronic treatments with aspirin or acetaminophen reduce both the development of polyarthritis and Fos-like immunoreactivity in rat lumbar spinal cord Abbadie, C.;Besson, J.M. https://doi.org/10.1016/0304-3959(94)90106-6
  3. Clin Exp Immunol v.97 Role of cytokines in experimental arthritis Brennan, F.M.
  4. Nature v.245 Letter: An anti-inflammatory peptide from bee venom Billingham, M.E.;Morley, J.;Hanson, J.M.;Shipolini, R.A.;Vernon, C.A. https://doi.org/10.1038/245163a0
  5. Pain v.83 Primary hyperalgesia to mechanical and heat stimuli following subcutaneous bee venom injection into the plantar surface of hindpaw in the conscious rat: a comparative study with the formalin test Chen, J.;Luo, C.;Li, H.;Chen, H. https://doi.org/10.1016/S0304-3959(99)00075-5
  6. Biochem Pharmacol v.31 Effect of honeybee (Apis mellifera) venom on the course of adjuvant- induced arthritis and depression of drug metabolism in the rat Eiseman, J.L.;von Bredow, J.;Alvares, A.P. https://doi.org/10.1016/0006-2952(82)90354-9
  7. Arthritis Rheum v.38 Measurement of radiographic changes in adjuvant-induced arthritis in rats by quantitative image analysis Esser, R.E.;Hildebrand, A.R.;Angelo, R.A.;Watts, L.M.;Murphey, M.D.;Baugh, L.E. https://doi.org/10.1002/art.1780380120
  8. J Rheumatol v.11 Bee venom and adjuvant induced disease Hadjipetrou-Kourounakis, L.;Yiangou, M.
  9. Agents Actions v.34 Comparison of antiinflammatory and antiallergic drugs in the melittin-and D49 PLA2-induced mouse paw edema models Hartman, D.A.;Tomchek, L.A.;Lugay, J.R.;Lewin, A.C.;Chau, T.T.;Carlson, R.P. https://doi.org/10.1007/BF01993245
  10. Acta Physiol Pharmacol Bulg v.11 Further investigation on the antiinflammatory properties of adolapin-- bee venom polypeptide Koburova, K.L.;Michailova, S.G.;Shkenderov, S.V.
  11. Pain v.84 Computer-assisted infrared thermographic study of axon reflex induced by intradermal melittin Koyama, N.;Hirata, K.;Hori, K.;Dan, K.;Yokota, T. https://doi.org/10.1016/S0304-3959(99)00192-X
  12. Pain Bee venom injection into an acupuncture point reduces arthritis associated edema and nociceptive responses Kwon, Y.B.;Lee, J.D.;Lee, H.J.;Han, H.J.;Mar, W.C.;Beitz, A.I.;Lee, J.H.
  13. Toxicon v.38 Effect of crotapotin and heparin on the rat paw oedema induced by different secretory phospholipases A2 Landucci, E.C.;Toyama, M.;Marangoni, S.;Oliveira, B.;Cirino, G.;Antunes, E.;de Nucci G. https://doi.org/10.1016/S0041-0101(99)00143-9
  14. Pain v.66 The bee venom test: a new tonic-pain test. Lariviere, W.R.;Melzack, R. https://doi.org/10.1016/0304-3959(96)03075-8
  15. Pain v.63 Capsaicin-induced flare and vasodilatation in patients with post-herpetic neuralgia Morris, G.C.;Gibson, S.J.;Helme, R.D. https://doi.org/10.1016/0304-3959(95)00019-O
  16. Am J Physiol v.273 Relations between functional, inflammatory, and degenerative parameters during adjuvant arthritis in rats Philippe, L.;Gegout-Pottie, P.;Guingamp, C.;Bordji, K.;Terlain, B.;Netter, P.;Gillet, P.
  17. Pain v.16 Ethical guidelines for investigations of experimental pain in conscious animals Zimmermann, M. https://doi.org/10.1016/0304-3959(83)90201-4