Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effects of Puerariae flos herb-acupuncture on cell proliferation and neurogenesis in the dentate gyrus of ethanol-induced Sprague-Dawley rats

갈화 약침이 알콜 중독 흰쥐의 치상회에서 신경세포 생성에 미치는 영향

  • Kim Youn-Hee (Departments of Chemistry, College of Oriental Medicine, Se-Myung University) ;
  • Kim Ee-Hwa (Departments of Meridianology, College of Oriental Medicine, Se-Myung University) ;
  • Jang Mi-Hyun (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Lim Back-Vin (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Kim Youn-Jung (Department of Physiology, College of Medicine, Kyung-Hee University) ;
  • Chung Joo-Ho (Departments of Pharmacology, College of Medicine, Kyung-Hee University) ;
  • Seo Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Kim Chang-Ju (Department of Physiology, College of Medicine, Kyung-Hee University)
  • 김연희 (세명대학교 한의과대학 생화학교실) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실) ;
  • 장미현 (경희대학교 의과대학 생리학교실) ;
  • 임백빈 (경희대학교 의과대학 생리학교실) ;
  • 김연정 (경희대학교 의과대학 생리학교실) ;
  • 정주호 (경희대학교 의과대학 약리학교실) ;
  • 서정철 (동의대학교 한의과대학 침구경혈학교실) ;
  • 김창주 (경희대학교 의과대학 생리학교실)
  • Published : 2001.12.20
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Abstract

The purpose of this study was to determine the effects of Puerariae flos herb-acupuncture on hippocampal neural cell proliferation. Sprague-Dawley rats were randomly assigned into 4 groups; control group, control with herb-acupuncture group, alcohol group, alcohol with herb-acupuncture group group. Control groups were received with NaCl, while alcohol intoxication groups were injected intraperitoneally with alcohol (2 g/㎏) twice per day for 3 days. Herb-acupuncture groups were injected on Zhongwan (CV 12) for 5 consecutive days. Bromo-deoxyuridine (BrdU) was injected into all animal per day for 5 days. For the detection of BrdU-positive cells in dentate gyrus of hippocampus, immunohistochemistry was performed. In alcohol group, a significant decrease in BrdU-positive cells was observed compared to control group. In alcohol with herb-acupuncture group, BruU-positive cells increased significantly compared to alcohol group. In conclusion, the present results revealed that new cell proliferation is enhanced in the dentate gyros of young Sprague-Dawley rats through Puerariae flos herb-acpuncuture in an acute alcoholic intoxication condition.

Keywords

References

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