Effect ot Radix Asteris on Airway Smooth Muscle

Airway Smooth Muscle에 미치는 자울의 효과

  • Na Kyung-Sang (Dept. of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Eui-Kwang (Dept. of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Soo Yeung-Hyung (Dept. of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Suh Eun-Mi (Dept. of Pharmacology, College of Oriental Medicine, Wonkwang University) ;
  • Han Jong-Hyun (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 나경상 (원광대학교 한의과대학 약리학교실) ;
  • 권의광 (원광대학교 한의과대학 약리학교실) ;
  • 소응향 (원광대학교 한의과대학 약리학교실) ;
  • 서은미 (원광대학교 한의과대학 약리학교실) ;
  • 한종현 (원광대학교 한의학전문대학원)
  • Published : 2001.03.27

Abstract

Radix Asteris has been used in Korea for many centuries as a treatment for respiratory disease. The effect of Radix Asteris on tracheal smooth muscle is not known. The purpose of the present study is to determine the effect of Radix Asteris on histamine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats (250g, male) were killed by $CO_2$ exposure and a segment (8-10mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine (His) which evoked 50% of maximal response ($ED_{50}$) was obtained from cumulative dose response curves for histamine $(10^{-7}{\sim}10^{-4}M)$. Contractions evoked by His ($ED_{50}$) were inhibited significantly by Radix Asteris. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 120.5% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. In rat tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 135.4% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. Propranolol $(10^{-7}M)$ slightly but significantly attenuated the inhibitory effects of Radix Asteris. Following treatment with propranolol, the mean percent inhibition caused by $100{\mu}l/ml$ Radix Asteris fell to 44.6% in guinea pig induced by histamine contraction and by $100{\mu}l/ml$ Radix Asteris fell to 18.7% (p<0.05) in rat induced by histamine contraction. Indomethacin and methylene blue$(10^{-7}M)$ did not significantly alter the inhibitory effect of Radix Asteris. These results indicate that Radix Asteris can relax histamine induced contraction of guinea pig and rat tracheal smooth muscle, and that this inhibition involves sympathetic effects.

Keywords

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