The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Sintongchukeo-tang on the Cultured Spinal Sensory Neurons Injured by Hydrogen Peroxide

신통축어탕(身痛逐瘀湯)이 Hydrogen Peroxide에 의해 손상(損傷)된 배양(培養) 척수감각신경세포(脊髓感覺神經細胞)에 미치는 영향(影響)

  • Lee, Kye-Seung (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Na, Young-Hoon (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Cha, Yong-Suk (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Heo, Yun (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Do-Hwan (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Han, Sang-Hyok (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Park, Byong-Min (Dept. of Respiratory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Lee, In (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Moon, Byung-Soon (Dept. of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univ.)
  • 이계승 (원광대학교 한의과대학 심계내과학교실) ;
  • 나영훈 (원광대학교 한의과대학 심계내과학교실) ;
  • 차용석 (원광대학교 한의과대학 심계내과학교실) ;
  • 허윤 (원광대학교 한의과대학 심계내과학교실) ;
  • 김도환 (원광대학교 한의과대학 심계내과학교실) ;
  • 한상혁 (원광대학교 한의과대학 심계내과학교실) ;
  • 박병민 (원광대학교 한의과대학 폐계내과학교실) ;
  • 이인 (원광대학교 한의과대학 심계내과학교실) ;
  • 문병순 (원광대학교 한의과대학 심계내과학교실)
  • Published : 2001.12.30
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Abstract

Objectives : This study was carried out to examine toxic effect of Sintongchukeo-tang on cultured mouse spinal sensory neurons inhibited by neurotoxicity induced by hydrogen peroxide. Methods : MTT assay, NR assay, LDH and neurofilament assay were performed after spinal sensory neurons were preincubater with various concentrations of Sintongchukeo-tang water extract before treatment of cells with hydrogen peroxide. Results : Hydrogen peroxide induced ceil degeneration such as the decrease of cell viability was measured by MTT and NR assay in the cultured mouse spinal sensory neurons. Sintongchukeo-tang water extract was effective in the decrease of LDH activities of neurons produced by hydrogen peroxide. Sintongchukeo-tang water extract was effective in the increase of amount of neurofilaments damaged by hydrogen peroxide. Conclusions : From the above results, it is suggested that hydrogen peroxide induces the inhibition of cell viability in cultured mouse spinal sensory neurons and Sintongchukeo-tang water extract was effective in cultured neurons damaged by hydrogen peroxide.

Keywords

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