The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Studies on Submerged Culture and Mycelial Components of Naematoloma sublateritium Mycelia

개암버섯균의 액체배양과 균사체의 성분에 관한 연구

  • Kang, An-Seok (Kangwon Provincial Rural Development Administration) ;
  • Kang, Tae-Su (Department of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology) ;
  • Cho, Soo-Muk (National Institute of Agricultural Science and Technology, RDA) ;
  • Yu, Seung-Hun (Department of Agricultural Biology, Chungnam National University)
  • 강안석 (강원도 농업기술원 북부농업시험장) ;
  • 강태수 (도립충북과학대학 식품생명과학과) ;
  • 조수묵 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 유승헌 (충남대학교 농생물학과)
  • Published : 2001.04.30
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Abstract

This study was carried out to get the basic information for the submerged culture and analyze the biochemical components of Naematoloma sublateritium mycelia. The optimal temperature, pH, agitation speed and cultural time for the mycelial growth of Naematoloma sublateritium were $25^{\circ}C$, 5.5, 150rpm and 20 days, respectively. The proximate composition of mycelia was as follows; carbohydrate 55.8% (total sugar 48.7%), crude protein 22.4%, fat 4.1 % and ash 4.7% respectively. Among the free amino acid contents, phenylalanine, alanine and lysine were predominant component. The linoleic acid and palmitic acid were found to be the highest among the free fatty acids. The biopolymer extracts of mycelia was identified to be protein-bounded polysaccharide by color reaction and sepharose CL-4B gel chromatography.

본 연구는 개암버섯의 연주에 대한 기초자료를 마련하고자 개암버섯 균사체의 액체배양 조건과 성분을 분석하였다. 액체배양시 개암버섯 균사체의 생육최적 온도, pH 및 교반속도는 각각 $25^{\circ}C$, pH 5.5 및 150rpm이었으며, 배양기간은 20일이 좋았다. 균사체의 일반성분은 탄수화물 55.8%. 조단백질 22.4%,지방 회분이 각각 4.1%, 4.7%이었고, 전당함량이 48.7%이었다 균사체의 유리아미노산 함량은 phenylalanine, alanine 및 lysine이 많이 함유되어 있었고, 유리지방산으로는 linoleic acid와 palmitic acid가 많았다. 균사체 고분자 추출물을 발색반응과 Sepharose CL-4B를 이용한 겔크로마토그라피에 의해 정제한 결과, 주분획은 단백다당으로 판단되었다.

Keywords

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