Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effect of Carthami-Flos aquacupuncture on t-Butylhydroperoxide- induced inhibition of Na+-K+-ATPase activity in cerebral synaptosomes

홍화약침액(紅花藥鍼液)이 t-Butylhydroperoxide에 의한 가토(家兎)의 뇌조직(腦組織) Na+-K+-ATPase 활성장애(活性障碍)에 미치는 영향(影響)

  • Kim, Cheol-Woong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Youn, Hyoun-Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Jang, Kyung-Jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University) ;
  • Ahn, Chang-Beohm (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Eui University)
  • 김철웅 (동의대학교 한의과대학 침구경혈학교실) ;
  • 서정철 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실)
  • Received : 2001.03.08
  • Accepted : 2001.03.22
  • Published : 2001.04.20
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Abstract

Objectives ; This study was undertaken to determine whether Carthami-Flos aquacapuncture (CFA) exerts protective effect against oxidant-induced inhibition of $Na^+-K^+$-ATPase activity in cerebral synaptosomes. Methods and Results ; The enzyme activity was dependent on incubation time and enzyme protein concentrations. An oxidant t-butylhydroperoxide (tBHP) at 1 mM concentration caused a significant inhibition of $Na^+-K^+$-ATPase activity, which was prevented by addition of 0.01% CFA. tBHP inhibition and CFA protection were independent on incubation time or enzyme protein concentrations. The enzyme activity was increased by ATP in a dose dependent manner. Effects of tBHP and CFA were not affected by ATP cocentrations. tBHP (1 mM) produced a significant increase in lipid peroxidation in cerebral synaptosomes, which was prevented by 0.01% CFA. CFA decreased oxygen free radicals generated induced by the phorbol-ester in a dose-dependent manner in human neutrophil. Conclusions ; These results suggest that CFA exerts protective effect against tBHP-induced inhibition of $Na^+-K^+$-ATPase activity, which is due to by an antioxidant action resulting from a direct scavenging effect of oxygen free radicals in the cerebral synaptosomes.

Keywords

References

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