In this study, wool, silk and cotton fabrics were dyed with natural dyes derived from Ginkgo biloba bark using various mordants, and their dyeabilities were discussed. Additionally the fastness to washing, perspiration, light, rubbing, and drycleaning were investigated. And the effects of bacteria reduction and UV-B protection rate were also checked. The optimum dyeing condition of the colorants extracted from the Ginkgo biloba bark was three repeated dyeing at 95℃ for 1 hr. by using post mordanting. Mordanting improved the fastness to washing, Perspiration and drycleaning, but the fastness to light and rubbing were not increased. The bacteria reduction rate of the wool fabric increased drastically by dyeing with extract of Ginkgo biloba bark and its effect maintained after repeated washing and drycleaning. UV-B protection rate of the natural fibers increased by dyeing with extract of Ginkgo biloba bark and the dyed wool fabric was the best of the three fabrics.