Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effect of Scutellaria baicalensis Georgi Aquacupuncture on Oxidant-induced Cell Injury in Renal Cortical Slices

황금약침액(黃芩藥鍼液)이 신장조직(腎臟組織)에서 Oxidant에 의한 세포손상(細胞損傷)에 미치는 영향(影響)

  • Heo, Kyoung-Mee (Department of AM-Meridian & Pointology, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Department of AM-Meridian & Pointology, College of Oriental Medicine, Dong-Eui University)
  • 허경미 (동의대학교 한의과대학 경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 경혈학교실)
  • Received : 2001.03.06
  • Accepted : 2001.03.21
  • Published : 2001.04.20
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was undertaken to determine if Scutellaria baicalensis Georgi (SbG) extract exerts protective effect against oxidant-induced cell injury in renal proximal tubular cells. Methods : The cell injury was evaluated by lactate dehydrogenase (LDH) release in rabbit renal cortical slices and lipid peroxidation was estimated by measuring malondialdehyde (MDA). t-Butylhydroperoxide (tBHP) was used as a model of oxidant. Results : tBHP at 1 mM increased LDH release and lipid peroxidation, which were prevented by SbG in a dose dependent manner over concentration range of 0.001-0.1%. SbG provided the protective effect against oxidant-induced reduction in PAH uptake by renal cortical slices and microsomal Na+-K+-ATPase activity. SbG attenuated tBHP-induced depletion of reduced glutathione. 0.2 mM $HgCl_2$ increased LDH release and lipid peroxidation, which were completely prevented by 0.05% SbG. Conclusion : SbG prevents oxidant-induced impairment in membrane transport function.

Keywords

References

  1. Chem Pharm Bull. v.36 Two new flavonoidand other constituents in licorice root: their relative astringency and radical scavenging effects. Hanato, T.;Kagawa, H.;Yasuhara, T.;Okuda, T.
  2. FASEBJ. v.4 Role of oxygen free radical in carrion genesis and brain ischemia. Floyd, R.A.
  3. Kid Int. v.40 Reactive oxygen species and ratrenalepithelial cells during Hypoxia and reoxygenation Palier, M.S.;Neumann, T.V.
  4. Am J Physiol v.251 Reactive oxygen species : production and role in the kidney Baud, L.;Ardaiilou, R.
  5. Kidney Int. v.29 A role for oxygen free radicals in aminonucleoside nephrosis Diamond, J.R.;Bonventre, J.V.;Karnovsky, M.J.
  6. Curr Opin Nephrol Hypertens. v.2 Postischemic renal injury due to oxygen radicals. Johnson, K.J.;Weinberg, J.M.
  7. 本草學 全國韓醫科大學 本草學敎室
  8. 大韓鍼灸學會誌 v.15 no.1 黃芩藥鍼液의 흰쥐 肝癌細胞의 抗酸化 效能에 관한 硏究 金性速;部垣錫;金甲成
  9. Anal Biochem. v.86 Determination of malonaldehydeprecusor intissue by thiobarbiturlc acid test Uchiyama, M.;Mihara, M.
  10. Anal Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the Principle of protein-dry binding Bradfordm, M.
  11. J Biol Chem. v.66 The colorimetric determination of phosphorus Fiske, C.H.;Subbarow, Y.
  12. Antioxidant aging and iongevity, Free Radicals in Biology Cutler, R.G.
  13. The free radical theory of aging, free radical reaction in medicine Feher. J.;Csomos, G.;Verekei, A.
  14. SOD and active oxygen moduiater Oyanagui, Y.
  15. FASEB J. v.9 Oxidative processes and antioxidative defense mechanism in the againg brain Heifer, R.J.
  16. J Lab ClinMed. v.119 Free radicals antioxidants and human disease. Where are we now?. Halliwell, B.;Gutteridge, J.M.C.;Cross, C.E.
  17. Physiol Rev. v.59 Hydroperoxide metabolism an mammalian organs. Chance, B.;Sies, H.;Boveris, A.
  18. Am J Physiol. v.254 Depression of membrane –bound $Na^+$-$K^+$-ATPaseactivityinducedby free radical and by ischemia of kidney Kako, K.;Kato, M.;Matsuoka, T.;Mustapha, A.
  19. J Gerontol. v.23 Free radical theory of aging. Harman, D.
  20. 洛性酸素가 질병의 원인이다 오유진
  21. 지방질대사 蔡範錫
  22. Lab Invest. v.62 Biology of disease. Mechanisms of cell injury by activated oxygen species Farber, J.L.;Kyle, M.E.;Coleman, J.B.
  23. Am J Physiol v.255 Mechansims of t-but -ylhydroperoxide-induced toxicity to rabbit renal proximal tubules Schnellmann, R.G.
  24. The pharmacology of mercury compounds. Ann Rev pharmacol. v.12 Clarkson TW.;Elliott, H.W.;Okun, R.;George, R.(Ed.)
  25. Ann Intern Med. v.76 Heavy metal poisoning, mercury and lead. Felton, J.S.;Kahn, E.;Salick, B.;Van Natta, F.C.;Whitehouse, M.W.
  26. Toxic effect of metals, the basic science of poisoning Coyer, R.A.;Amder, M.O.;Douli, J.;Klassen, C.D.(Ed.)
  27. J Ultrastruct Res. v.3 The localization of subcutaneously administered radio active mercury in the rat kidney. Bergstrand, A.;Friberg, L.;Mendel, L.;Odeblad, E.
  28. Am J Physiol. v.213 Organic mercurial and net movement of potassium in rat kidney slices. Bowman, F.J.;Landon, E.J.
  29. 생리학 養樹寬;全世烈
  30. 東國論集 自然科學編 v.15 柴胡가 free radical에 의한 지질과산 화물 생성에 미치는 영향 문진영;최미정;남경수;임종국
  31. 生理學 李炳熙
  32. 臨床配合本草學 姜秉秀;金永板
  33. 생리학회지 v.25 no.1 黃芩의 Flavonoid 성분들이 Rat 수정체의 백내장 형성과 Polyol측적에 미치는 영향 신국현;채윤정;정명숙;이희주
  34. 黃帝內經素問解釋 南京中醫學院醫經敎硏(組)
  35. 醫學正傳 虞搏
  36. 東醫腎系內科學 杜鎬京
  37. 대한한의학회지 v.17 no.1 左歸飮과 右歸飮에 의한 활성산소류의 消去作用과 拓酸化酸素系의 활성증가효과에 대한 연구 鄭智天
  38. 대한한의학회지 v.17 no.1 人工膜과 Rat의 肝細胞를 이용한 血府逐瘀湯의 항산화 작용에 관한 연구 禹大潤;李泰均;文振榮;林種國
  39. Envirpon Health Perspect v.15 Differential effects of nophrotoxic agents on renal transport and metabolism by use of In vitro techniques. Hirsch, F.H.
  40. 프리라디칼 김영곤(외)