Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Distribution of Extracellular Proteases from Various Vibrio Species

비브리오속 균주들에서외 세포의 효소의 분포

  • 차재호 (부산대학교 미생물학과) ;
  • 김윤희 (부산대학교 미생물학과) ;
  • 정초록 (부산대학교 미생물학과) ;
  • 김수광 (부산대학교 미생물학과) ;
  • 양지영 (부경대학교 식품생명공학부)
  • Published : 2001.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The members of the genus Vibrio include harmless aquatic strain as well as strains capable of causing infections in human and fish. Pathogenic mechanisms are only understood for Vibrio cholerae O1 and O139 and not for the majority of Vibrio species. Twelve clinical and nonclinical strains were examined by in vitro and in vivo experiments for the importance of extracellular enzymes as a virulence determinant of Vibrio species. In vivo cytotoxicity assay was performed by injecting approximately $10^{8}$ cells/mL into mice (BALB/c). V. harvyi and V. vulnificus showed 100% lethality within 3hr after bacterial injection. V. fluvialis and four strains of V. parahaemolyticus showed 50% lethality within 4hr. V. mimicus, V. alginolyticus and V. furnissii revealed 30% lethality within 9hr. Nonclinical strains, V. campbellii and V. ordalii, did not show any lethality. In vitro protease and hemolytic activities were also good indicators for clinical and nonclinical strains of Vibrio species. The clinical strains showed much higher activities than nonclinical strains. The activity of some clinical strains of re-isolates was evidently increased. Most clinical strains had $\beta$ hemolytic activity. The results demonstrate that the prevalent distribution of extracellular proteases in pathogenic Vibrio sp. implies their importance as a virulence determinant.

Keywords

References

  1. J.Infect.Dis v.173 The epidemiology of Vibrio infections in Florida Hiady,W.G;Klontz,K.C
  2. Vibrio vulnificus In Foodbornd Bacterial Path ogens Oliver,J.D;Doyle,M.P(ed)
  3. Manual of Clinical Microbiology(4th ed) Farmer,J.J.III;Hickman-Brenner,F.W;Kelly,M.T
  4. J Food Protect v.52 Preliminary estimates of costs of foodborne disease in the U S Todd,E C D
  5. Enteric gram negative microorganisms(14th ed) Jawetz.E;Melnick,J.L;Adelberg,E.A
  6. Host-microbe interactions: The process of infection.In Microbiology(5th ed) Pelezer,M.J;Chan,E.C.S;Krieg,N.R
  7. Microb Pathog v.23 Some properties of nicked Vibrio vulnificus hemolysin Myoshi,S;Fujii,S;Tochika,K;Shinoda,S
  8. J.Gen.Microbiol v.137 Characterization of a new thermostable direct hemolysin produced by a Kanagawa-phenomenon-negative clinical isolated of Vibrio parahaemolyticus Honda,T;Abad-Lapuebla,M.A;Ni,Y.X;Yamamoto,K;Miwatani,T
  9. FEMS Microbiol.Lett v.67 Comparative analysis of the haemolvsin genes of Vibrio cholerae non-O1,V mimicus and V.hollisae that are similar to the ldh gene of V.parahaemolytocus Nishibuchi,M;Khaeomanee-iam,V;Honda,T;Kaper,J.B;Miwatani,T
  10. J.Gen.Microbiol v.133 Monoclonal antibodies against the haemolysin of Vibrio vulnificus Okada,K;Mitsuyama,M;Miake,S;Amako,K
  11. Infect Immun v.49 Detection of the thermostable direct hemolysin gene and related DNA sequences in Vibrio parahaemolyticus and other Vibrio species by the DNA colony hybridization test Nishibushi,M;Ishibashi,M;Takeda,Y;Kaper,J.B
  12. J.Gen.Microbiol v.139 Rapid and specific detection of the thermostable direct haemolysin gene in Vibrio parahaemolyticus by the polymerase chain reaction Lee,C;Pan,S.F
  13. Infect Immun v.58 Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum Norqvist.A;Nortman,B;Wolf-Watz,H
  14. J.Infect Dis v.144 Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes association of virulence with resistance to phagocytosis Kreger,A;De Chatelel.L;Shirley,P
  15. J.Infect.Dis v.130 Extracellular toxins of Pseudomonas aeruginosa Liu,P.V
  16. Infect Immun v.53 Degradation of protease inhibitors,immunoglobulins and other serum proteins by Serratia protease and its toxicity to fibroblasts in culture Molla,A.Matsumoto;K.Oyamada,I;Katsuki,T;Maeda,H
  17. Microbial Pathogen v.28 Construction and virulence testing of a collagenase mutant of Clostridium perfringens Award,M.M;Ellemor,D.M;Bryant,A.E;Matsushita,O;Boyd,R.L;Stevens.D.L;Emmins,J.J;Rood,J.I
  18. FEMS Microbiol.Lett. v.77 Characterization of two forms of hemagglutinin/protease produced by Vibrio cholerae non-O1 Naka,A;Yamamoto,K;Miwatani,T;Honda,T
  19. Infect Immun v.60 Vibrio cholerae hemagglutinin/protease,colonial variation,virulence, and detachment Finkelstein,R.A;Boesman-Finkelstein,M;Chang,Y;Hase,C.C
  20. Infect Immun v.50 Production and partial characterization of an elastolytic prolease of Vibrio vulnificus Kothary,M.H;Kreger,A.S
  21. Infect Immun v.68 Construction and phenolypic evaluation of a Vibrio vulnificus vvpE mutant for elastolytic protease Jeong.K.C;Jeong,H.S;Rhee,J.H;Lee,S.E;Chung,S.S;Starks,A.M;Escudero,G.M;Gulig,P.A;Choi,S.H
  22. J Bacterial v.174 Colning of a metalloprotease genic involyed in the virulence mechanism of Vibrio anguillarum Milton,D.L;Norqvist,A;Wolf-Watz,H
  23. FEMS Microbiol Lett v.40 Virulence of Vibrio vulnificus:association with utilization of transferm-bound iron,and lack of correlation with levels of cytotoxin or protease production Morris,Jr,J G;Wright,A.C;Simpson,L.M;Wood,P.K;Johnson,D.E;Oliver,J.D
  24. J.Infect Dis v.145 Purification and characterization of the mucinase of Vibrio cholerae Schneider,D.R;Parker,C.D
  25. J.Med.Microbiol v.22 Studies on the Vibrio cholerae mucinase complex I.Enzymic activities associated with the complex Stewart-tull,D.E.S;Ollar,R.A;Scobie,T.S
  26. Meth Enzymol v.235 Assays for hyaluronidase activity Hynes,W.L;Ferretti.J.J
  27. J.Med Ver.Mycol v.33 Hyaluronidase and chondroitin sulfatase production by different species of Candida Shimizu,M.T;Jorge,A.O;Unterkircher,C.S;Fantinato,V;Paula,C.R
  28. Appl.Microbiol Biotechnol v.41 Cloning and expression in Escherichia coli of the gene encoding the Proteus vulgaris chondroitin ABC lyase Sato,N;Shimada,M;Nakajima,H;Oda,H;Kimura,S
  29. Microbiol Immunol v.31 Chondroitin sulfate-depolymerizing activity in Streptococcus intermedius and other Streptococci Osano,E;Ijibi,E;Ozeki,M;Fujii,Y;Moriyama,T
  30. J.Gen Microbiol v.133 Purfication and characterization of an elastolytic protease of Vibrio vulnificus Kothary,M.H;Kreger,A.S
  31. Pharmacol.Ther v.19 Thermostable direct hemolysin of Vibrio parahaemolyticus Takede,Y
  32. Comp Biochem.Physiol. v.125 Coagulation and fibrinolysis in capyhara, a close relative of the guinea-pig Leitao,D.P.S;Polizello,A.C.M;Rothscuild,Z
  33. Diagn.Microbial.Infect.Dis v.5 Production of extracullular enzymes and cytotoxicity by Vibrio vulnificus Oliver,J.D;Wear,J.E;Thomas,M.B;Warner,M;Linder,K
  34. J.Clin Microbiol v.19 Comparative studies and laboratory diagnosis of Vibrio vulnificus, an invasive Vibrio sp Desmond,E.P;Janda,J.M;Adams,F.I;Bottone,E.J