Antioxidative Activity of Extrcts from Fruit of Curdrania tricuspidata

꾸지뽕나무 열매 추출물의 항산화 활성

  • 차재영 (동아대학교 생명자원과학부) ;
  • 조영수 (동아대학교 생명자원과학부)
  • Published : 2001.06.01

Abstract

The comparative activities of acetone, ethanol, and aqueous fractions extracted from fruit powder of Cudrania tricuspidata by different temperature were tested by in vitro experimental models; peroxidation of linoleic acid and autooxidation of rat hepatic and renal microsomes by using thiobarbituric acid (TBA) for assay of free malondialdehyde production, and scavenging activities of free radicals by DPPH (α, α'-diphenyl-β-picrylhydrazyl). In DPPH method, acetone fraction extracted at 30℃ showed the highest free radical scavenging activities and acetone fractions extracted at 30℃ and 60℃ and ethanol fraction extracted at 30℃ showed stronger than BHT (butylated hydroxitoluene) although used ten-fold lower concentrations. In thiocyanate method used linoleic acid an inhibitory effects of all fractions showed higher than control treatment. TBA method used linoleic acid showed the highest antioxidative activity in acetone fraction extracted at 30℃ and 60℃. an inhibition activity against lipid peroxidation in hepatic microsomes of rats showed the highest at acetone faction extracted at acetone fraction among extracted fractions was shown to be the most potent antioxidative properties and this action was more potent in fractions extracted at 30℃ than those extracted at 60℃.

Keywords

References

  1. Dehanshilkmuldogam Lee, C B
  2. Jungyakdesajon(2nd) Kangsoshinuihakwon
  3. Planta Medica v.51 Components of root bark of Cudrania tricuspidata 3. Isolation and structure studies on the flavonoids Fujimoto, T.;Nomura, T.
  4. Yakhak Hoeji v.36 Constituents of Cudrania tricuspidata in Korea Pack, I.C;Young, H.S.;Choi, J.S.
  5. J. Korean Soc. Food Sci. Nutr. v.22 Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau Kim, S.H.;Kim, N.J.;Choi, J.S.;Park, J.C
  6. Acta Chem. Scand [B] v.31 The crystal structure of cudranone, 2,6,3'-trihydroxy-4-methoxy-2'-(3-methyl-2-butenyl)-Ⅰ.a new antimicrobial agent from Cudrania chochinchinensis Ottersen, T;Vance, B.;Doorenbos, N J.;Chang, B. L.;El-Feraly, F S
  7. J. Ethnopharomacol. v.44 Effects of anti-lipid peroxidation of Cudrania cochinchinensis var gerontogea Chang, C.H.;Lin, C.C.;Hattori, M.;Namba, T.
  8. J Korean Soc. Food Sci. Nutr v.28 Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata Cha, J.Y.;Kim, H.J.;Chung, C H.;Cho, Y S.
  9. J. Korean Soc. Food Sci. Nutr. v.29 Effect of watersoluble extract from leaves of Morus alba and Cudrania tricuspidata on the lipid peroxidation in tissue of rats Cha, J.Y;Kim, H.J.;Cho, Y.S.
  10. J Korean Soc. Agric. Chem. v.43 Antioxidative activities by water-soluble extracts of Morus alba and Cudrania tricuspidata Kim, H.J;Cha, J Y;Choi, M.R.;Cho, Y.S.
  11. Agric. Chem Biotechnol. v.43 Effect of water extract of leaves from Morus alba and Cudranta tricuspidata on the lipid concentration of serum and liver in rats Cha, J.Y.;Kim, H.J;Jun, B.S.;Cho, Y.S.
  12. J Korean Soc. Food Sci. Nutr. v.27 Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats Kim, S Y;Lee, W.C;Kim, H.B;Kim, A.J.;Kim, S.K.
  13. Korean J. Food Sci. Thechnol v.33 Effect of stem bark extract from Morus alba and Cudrania tricuspidata on the concentrations of lipid and tissue lipid peroxidation in the cholesterol-fed rats Cha, J Y;Cho, Y S.
  14. J. Korean Soc. Food Nutr. v.27 Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of saffloer (Carthamus tinctorius L.) Kim, H.J.;Jun, B.S.;Kim, S.K.;Cha, J.Y.;Cho, Y.S.
  15. J Korean Soc. Food Nutr. v.28 Effect of potato polyphenolics on lipid peroxidation in rats Cha, J Y;Cho, Y S.
  16. Kor. J. Pharmacogn. v.26 Effects of the fraction from the leaves, fruits, stems and roots of Cudrania tricuspidata and flavonoids on lipid peroxidation Park, J.C.;Choi, J.S.;Choi, J W.
  17. Nature v.26 Antioxidant determination by the use of a stable free radical Blois, M.S.
  18. Agric. Biol. Chem. v.45 A novel type of antioxidant isolated from leaf of Eucalyptus leaves Osawa, T.
  19. Arch. Biochem Biophys. v.79 Interaction of ascorbic acid and mitochondrial lipids Ottolenghi, A
  20. J Inorganic. Biochem. v.14 The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid Wong, S F;Holliwell, B;Richimond, R.;Skowroneck, W.R.
  21. Korean J. Food Sci. Technol. v.27 Antioxidative effects of soybean extracts by using various solvents Kim, J.Y.;Maeng, Y S.;Lee, K.Y.
  22. SOD and active oxygen modulators Oyanagui, Y
  23. Robbins pathologic basis of disease Corfrau, R.S.;Kumar, V;Robbins, S.L.;Saunders, W.B.
  24. Annu. Rev. Pharmacol. Toxical. v.16 Chemicals, drugs and lipid peroxidation Plaa, G.L.;Witschi, H.
  25. Free radicals, aging and degenerative disease Johnson, J.E.;Walford, R.;Harma, D.;Miquel, J.;Alen R.
  26. Planta Medica. v.51 Components of root bark of Cudrania tricuspidata 3 Isolation and structure studies on the flavonoids Fujimoto, T;Nomura, T.
  27. Am J Kidney Disease v.23 Flavonoid-induced acute nephropathy Lin, J.L.;Ho, Y.S.