Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Fed-batch Fermentation for Production of Nitrile Hydratase by Rhodococcus rhodochrous M33

  • Kim, Bu-Youn (R&D Center, Tong Suh Petrochemical Corp.Ltd.) ;
  • Kim, Jong-Chul (R&D Center, Tong Suh Petrochemical Corp.Ltd.) ;
  • Lee, Hyune-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Hyun, Hyung-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • Published : 2001.01.01
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Abstract

To enhance the productivity and activity of nitrile hydratase in Rhodococcus rhodochrous M33, a glucose-limited fed-batch culture was performed. In a fed-batch culture where the glucose was controlled at a limited level and cobalt was supplemented during the fermentation period, the cell mass and total activity of nitrile hydratase both increased 3.3-fold compared to that in the batch fermentation. The productivity of nitrile hydratase also increased 1.9-fold compared to that in the batch fermentation. The specific activity of nitrile hydratase in the whole cell preparation when using a fed-batch culture was 120 units/mg-DCW, which was similar to that in the batch culture.

Keywords

References

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