Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Sequencing of the ${\beta}-Amylase$ Gene from Paenibacillus sp. and Its Expression in Saccharomyces cerevisiae

  • Jeong, Tae-Hee (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Kim, Hee-Ok (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Park, Jeong-Nam (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Lee, Hye-Jin (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Shin, Dong-Jun (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Lee, Hwang-Hee Blaise (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Chun, Soon-Bai (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University) ;
  • Bai, Suk (Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University)
  • Published : 2001.02.01
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Abstract

A gene from Paenibacillus sp. KCTC 8848P encoding ${\beta}-amylase$ was cloned and expressed in Escherichia coli. The Paenibacillus ${\beta}-amylase$ gene cosisted of a 2,409-bp open reading frame without a translational stop codon, encoding a protein of 803 amino acids. The presumed ribosime-binding site, GGAGG, was located 10 bp upstream from the TTG initiation codon. The deduced amino acid sequence of the ${\beta}-amylase$ gene had a 95% similarity to the ${\beta}-amylase$ of Bacillus firmus. The ${\beta}-amylase$ gene was introduced into wild-type strains of Saccharomyces cerevisiae using a linearized yeast integrating vector containing a geneticin resistance gene and its product was secreted into the culture medium.

Keywords

References

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