Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Prediction of the Secondary Structure of the AgfA Subunit of Salmonella enteritidis Overexpressed as an MBP-Fused Protein

  • Won, Mi-Sun (Genome Research Center, Korea Research institute of Bioscience and Biotechnology) ;
  • Kim, So-Youn (Department of Food Science and Technology Chungnam National University) ;
  • Lee, Seung-Hwan (Department of Food Science and Technology Chungnam National University) ;
  • Kim, Chul-Jung (Department of Verterinary Medicine, Chungnam National University) ;
  • Kim, Hyun-Su (Department of Verterinary Medicine, Chungnam National University) ;
  • Jun, Moo-Hyung (Department of Verterinary Medicine, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science and Technology Chungnam National University)
  • Published : 2001.02.01
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Abstract

To examine the characteristics of the recombinant thin aggregative fimbriae of Salmonella, the AgfA subunit gene was amplified from Salmonella enteritidis using a PCR. The maltose binding protein (MBP)-AgfA fusion protein was overproduced in E. coli and purified. The secondary structure of AgfA was then elucidated from the difference CD spectra. An estimation of the secondary structure of AgfA using the self-consistent method revealed a mostly ${\beta}-sheet$ structure.

Keywords

References

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