Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Development of High Performance Liquid Chromatography for Paclitaxel Purification from Plant Cell Cultures

  • Published : 2001.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Paclitaxel can be produced in high yield and with a high degree of purify from plant cell cultures of Taxus chinensis. The complete purification method was systematically established and described. This method was an efficient procedure for the purification of paclitaxel from crude paclitaxel, consisting or reverse-phase chromatography, followed by a normal-phase chromatography. The two-stage HPLC purification scheme serves as an effective and economical approach for resolving paclitaxel from complex mixtures of taxoids, with high purify (>99%) and low impurities (<0.1%). The process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. The process has been optimized to minimize solvent usage, complexity, and operating costs.

Keywords

References

  1. J. Liq. Chromatogr v.14 HPLC separation of taxol and cephalomannine Cardellina Ⅱ, J. H.
  2. U.S. Patent 5,549,830 Reverse osmosis and ultrafiltration methods for solutions to isolate desired solutes including Taxane Carver, D. R.;T. R. Prout;C. T. Workman;C. L. Hughes
  3. J. Liq. Chromatogr. v.16 Determination of taxol in taxus media needles in the presence of interfering compounds Castor, T. P.;T. A. Tyler
  4. U.S. Patent 5,750,709 Method and apparatus for isolating therapeutic compositions from source materials Castor, T. P.
  5. U.S. Patent, 5,871,979 Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture Choi, H. K.;T. L. Adams;R. W. Stahlhut;S. I. Kim;J. H. Yun;B. K. Song;J. H. Kim;S. S. Hong;H. S. Lee
  6. J. Microbiol. Biotechnol. v.9 Cultivation of Digitalis lanata cell suspension in an aqueous two-phase system Choi, Y. S.;S. Y. Lee;D. I. Kim
  7. U.S. Patent 5,618,538 Methods and compositions for isolating taxanes Elsohly, H. N.;E. M. Jr. Croom;M. A. ElSohly;J. D. McChesney
  8. U.S. Patent, 5,900,979 Method for purifying taxol from Taxus biomass Hong, S. S.;B. K. Song;J. H. Kim;C. B. Lim;H. S. Lee;K. W. Kim;I. S. Kang;H. B. Park
  9. J. Nat. Prod. v.57 New bioactive taxoids from cell cultures of Taxus baccata Ma, W.;G. L. Park;G. A. Gomez;M. H. Nieder;T. L. Adams;J. S. Aynsley;O. P. Sahai;R. J. Smith;R. W. Stahlhut;P. J. Hylands
  10. U.S. Patent 5,478,736 Process for the isolation and purification of taxol and taxanes from Taxus using methanol or acetone Nair, M. G.
  11. U.S. Patent 5,670,673 Method for the isolation and purification of taxol and its natural analogues Rao, K. V.
  12. Pharm. Res. v.12 A new large-scale process for taxol and related taxanes from Taxus brevifolia Rao, K. V.;J. B. Hanuman;C. Alvarez;M. Stoy;J. Juchum;R. M. Davies;R. Baxley
  13. J. Microbiol. Biotechnol. v.8 Paclitaxel (Taxol): From nutt to drug Sohn, H.;M. R. Okos
  14. Appl. Biochem. Biotechnol. v.54 Taxol (Paclitaxel), strategies to increase the supply of a new anticancer drug Stull, D. P.;T. A. Scales;R. Daughenbaugh;N. A. Jans;D. T. Bailey
  15. J. Liq. Chromatogr. v.15 High-speed countercurrent chromatography separation of taxol and related diterpenoids from Taxus baccata Vanhaelen-Fastre, R.;B. Diallo;M. Jaziri;M. L. Faes;J. Homes;M. Vanhaelen
  16. J. Nat. Prod. v.53 Taxus spp. needles contain amounts of taxol comparable to the bark of Taxus brevifolia: Analysis and isolation Witherup, K. M.;S. A. Look;M. W. Stasko;T. J. Ghiorzi;G. M. Muschik
  17. J. Chromatogr.A v.855 Optimization of throughput and desorbent in SMB chromatography for paclitaxel purification Wu, D. J.;Z. Ma;N.-H. L. Wang
  18. Phytochem v.30 Taxanes from Taxus chinensis Zhang, Z.;Z. Jia