Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Pilot-Scale Production of Cellulase Using Trichoderma reesei Rut C-30 Fed-Batch Mode

  • Lee, Sang-Mok (Department of Biological Engineering, Inha University) ;
  • Koo, Yoon-Mo (Department of Biological Engineering, Inha University)
  • Published : 2001.04.01
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Abstract

Trichoderma reesei Rut C-30 produced high levels of ${\beta}$-glucosidase, endo-${\beta}$-glucosidase, endo-${\beta}$-1,4-glucanase, and exo-${\beta}$-1,4-glucanase. In pilot-scale production (50-1 fermentor), productivity and yield of CMCase (carborymethyl cellulose) and FPase (filter paper activity) were 273 U/ml and 35 U/ml, and 162 FPU/l.h and 437 FPU/g, respectively. The fed-batch techniques were used to improve enzyme activities with constant cell concentration. The acidity was an important parameter and controlled at pH 3.9 and 5.0 by automatic addition of ammonium hydroxide. Cellulase powder was prepared by ammonium sulfate precipitation and its CMCase and FPase activities were 3,631 U/g and 407 U/g, respectively.

Keywords

References

  1. Biotechnol. Bioeng. Symp. v.12 Cellulase production in continuous and fed-batch culture by Trichoderma reesei MCG80 Allen, A.;R. E. Andreotti
  2. Biotechnol. Bioeng. v.21 Production of cellulase by Trichoderma reesei QM9414 in fed-batch and continuous flow culture with cell recycle Ghose, T. K.;V. Sahai
  3. Pure Appl. Chem. v.59 Measurements of cellulase activities Ghose, T. K.
  4. Enzyme Microb. Technol. v.6 Enhanced cellulase production in fed-batch culture of Trichoderma reesei C-30 Hendy, N. A.;C. R. Wilke;H. W. Blanch
  5. Biotechnol. Lett. v.4 Enhanced cullulase production using Solka-Floc in a fed-batch fermentation Hendy, N. A.;C. R. Wilke;H. W. Blanch
  6. ACS Symp. Ser. 655 Roles for microbial enzymes in pulp and paper processing Kirk, T. K.;T. W. Jeffries
  7. Appl. Microbiol. v.43 Effect of agitation on growth and enzyme production of Trichoderma reesei in batch fermentation Lejeune, R.;G. V. Baron
  8. Annu. Reports Ferment. Process v.5 Cellulase Mandels, M.
  9. Process Biochem. v.12 Problems and challenges in the cellulose to cellulase to fermentation Mandels, M.;R. E. Andreotti
  10. Can. J. Chem. Eng. v.64 Fed-batch production of cellulase using Trichoderma reesei Rut C-30 Mclean, D. D.;K. Abear
  11. Biotechnol. Lett. v.7 Further support for fed-batch production of cellulases Mclean, D. D.;M. F. Podruzny
  12. Appl. Environ. Microbiol. v.34 Preparation of mutants of Trichoderma reesei with enhanced cellulase production Montenecourt, B. S.;D. E. Eveleigh
  13. Agric. Biol. Chem. v.49 Improvement of celllulase production in Trichoderma reesei Morikawa, Y.;M. Kawamori;Y. Ado;Y. Shinsha;F. Oda;S. Takasawa
  14. Biotechnol. Bioeng. v.22 Increased production of cellulase of Trichoderma sp. by pH cycling and temperarture profiling Mukhopadhyay, S. N.;R. K. Mailk
  15. Biochemistry and Genetics of Cellulose Degradation Scale up of cellulose production and urilization Pourquie, J.;M. Warzwoda;F. Chevron;M. Thery;D. Lonchamp;J. P. Vandecasteele;J. P. Aubert(ed.);P. Berguin(ed.);J. Milet(ed.)
  16. J. Gen. Appl. Microbiol. v.35 Cellulase production by partially catabolite resistant of Trichoderma reesei Rakshit, S. K.;V. Sahai
  17. Biotechnol. Bioeng. v.23 Enhanced production of cellulase, hemicellulase, and β-glucosidase by Trichoderma reesei (Rut C-30) Tangnu S. K.;W. B. Harvey;C. R. Wilke
  18. Biotechnol. Lett. v.6 Cellulase production by Trichoderma reesei (Rut C-30) in fed-batch culture Watson, T. G.;I. Neligan;L. Lessing
  19. Enzyme Systems for Lignocellulose Degradation Mechanisms of cellulose degradation by enzymes from aerobic and anaerobic fungi Wood, T. M.;M. P. Coughlan(ed.)
  20. Methods in Enzymology 160 Method for measuring cellulase activities Wood, T. M.;K. M. Bhat
  21. J. Microbiol. Biotechnol. v.9 Cellulase production in fed-batch culture by Trichoderma reesei Rut C-30 Yu, X. B.;H. S. Yun;Y. M. Koo
  22. J. Microbiol. Biotechnol. v.8 Production of cellulase by Trichoderma reesei Rut C-30 in a batch fermentor Yu, X. B.;H. S. Yun;Y. M. Koo