Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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High-Yield Production of Xylitol from Xylose by a Xylitol Dehydrogenase Defective Mutant of Pichia stipitis

  • Kim, Min-Soo (Department of Molecular Science and Technology, Ajou University) ;
  • Chung, Yun-Seung (Department of Food Science and Technology, Seoul National University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Seoul National University) ;
  • Jo, Do-Hyun (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Yun-Hee (Department of Molecular Science and Technology, Ajou University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University)
  • Published : 2001.08.01
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Abstract

This study was carried out in order to investigate the characteristics of xylitol fermentation by a xylitol dehydrogenase defective mutant PXM-4 of P stipitis CBS 5776 and to determime optimum conditions for the high yield ofxylitol production from xylose. Gluconic acid was selected as a co substrate for the xylitol fermentation, since gluconic acid neither blocked xylose transport nor repressed xylose reductase expression. An increase of gluconic acid concentration reduced the rates of xylitol production and cell growth by decreasing medium pH, and the optimal concentration of gluconic acid was determined to be 20 gll with approximately 100% xylitol conversion yield. A fed-batch cell culture resulted in a 44.8 g/l xylitol concentration with 100% yield, based on the amount of xylose consumed.

Keywords

References

  1. Appl. Environ. Microbiol v.54 Induction of xylose reductase and xylitol dehydrogenase activities in Pachsolen tannophilus and pichia stipitis Bicho, P.A.;P.L. Runnals;J.D. Cunningham;H. Lee
  2. Appl. Biochem. Biotechnol. v.45/6 The effect of cell density on the production of xylitol from Dxylose by yeast Cao, N.J.;R. Tang;C.S. Gong;L.F. Chen
  3. Biotechnol. Lett v.22 Production of xylitol in cell recycle fermentations of Candida tropicalis Choi, J.H.;K.W. Moon;Y.W. Ryu;J.H. Seo
  4. Can. J. Microbiol. v.37 Xylitol production by Petromyces albertensis grown on containing D-xylose Dahiya,J.S.
  5. Process Biochem v.29 Influence of oxygen on ethanol and xylitol production by xylose fermenting yeasts Furlan, S. A.;P. Bouilloud;H. F. de Castro
  6. J. Biochem v.225 The xylulose-xylitol enzyme and other polyol dehydrogenases of guinea pig liver mitochondria Hollmann, S.;O. Touster
  7. Adv. Food. Res. v.28 Food technological evaluation of xylitol Hyv$\"{o}$onen, L.;P. Koivistoinen;F. Voirol
  8. J. Ferment. Techol. v.66 production of xylitol from D-xylose by Mycobacterium smegmatis Ixumori, K.;K. Tuzaki
  9. J. Microbiol. Biotechnol. v.10 Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae containing genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis Jin, Y. S.;T,H. Lee;Y.D. Choi;Y. W. Ryu;J. H. Seo
  10. J. Ind. Microbiol. Biotechnol v.22 Analysis and optimization of a two-substrate fermentation for xylitol production using Candida tropicalis Kim, J. H.;Y. W. Pyu;J. H. Seo
  11. Kor. J. Biotechnol. Bioeng v.15 Enhancement of xylitol yield by xylitol dehydrogenase defective mutant of Pichia stipitis Kim, M. S.;C. Kim;J. H. Seo;Y. W. Ryu
  12. J. Ferment. Bioeng. v.83 Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis Kim, S.Y.;J. H. Kim;D.K. Oh
  13. Process Biochem v.35 Charcterization of two-substrate fermentation processes for xylitol production using recombinant Saccharomyces cerevisiae containing xylose reductase gene Lee, W.J.;Y.W. Ryu;J. H. Seo
  14. J. Microbiol Biotechnol v.9 Novel heterogeneous carbohydratase reaction systems for the direct conversion of insoluble carbohydrates: Reaction characteristics and their applications Lee, Y.H.;D.C. Park
  15. J. Biol. Chem. v.193 Protein measurement with the Folin-Phenol reagent Lowry, O. H.;N. J. Rosebrough;A. L. Farr;R. J. Randall
  16. US patent 4,0008,285 Process making for xylitol Melaja, A.;L. H$\"{a}$m$\"{a}$l$\"{a}$inen
  17. Adv. Food Res v.25 Xylitol and oral health M$\"{a}$kinen, K. K.
  18. J. Food Sci v.45 A quantitative study of mannitol, sorbitolm xylitol and xylose in wild berries and commercial fruits M$\"{a}$kinen, K.K.;E. S$\"{o}$derling
  19. J. Ferment. Bioeng. v.67 Conversion of D-xylose into xylitol by immobilized cells of Candida pelliculosa and Methanobacterium sp. HU. Nishio, N.;K. Sugawa;N. Hayase;N. Nagai
  20. Biotechnol. Lett. v.17 The reduction of xylose to xylitol by Candida guilliermondii and Candida parapsilosis; Incidence of oxygen pH. Nolleau, V.;L. Preziosi-Belly;J. M. Navarro
  21. J. Microbiol. Biotechnol. v.9 Mass production of poly(3-hydroxybutyrate)by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation Ryu. H.W.;K.S. Cho;B.S. Kim;Y.K. Chang;H.N. Chang;H. J. Shim
  22. Bioproc. Eng. v.11 Microbial production of xylitol from xylose using Candida tropicalis Silva, S.S.;A. S. Afschar
  23. J. Ferment. Bioeng. v.79 Screening of yeasts for production of xylitol from Dxylose Sirisansaneeyakul, S.;M. Kuzmanova;T.W. Jeffries
  24. Biotechnol. Lett. v.4 Demonstration of Dxylose reductase and D-xylitol dehydrogenase in Pachysolen tannophilus Smiley, K.L.;P.L. Bolen
  25. World J. Microbiol. Biothnol. v.11 Effects of environmental conditions on production of xylitol by Candida boidinii Vandeska, E.;S. Amartey;S. Kuzmanova;T.W. Jeffries
  26. J. Ferment. Bioeng v.67 Xylitol formation and key enzyme activities in C. boidinii under different oxygen transfer rate Vandeska, E.;S. Kuzmanova;T. W. Jeffries
  27. J. Microbiol. Biotechnol v.6 Characterization of alcohol fermentation and segregation of protoplast fusant of Saccharomyces cerevisiae and Pichia stipitis Yoon, G. S.;T.S. Lee;C. Kim;J.H. Seo;Y.W. Ryu
  28. J. Microbiol. Biotechnol v.9 Cellulase production in fed-batch culture by Trichoderma reesei Rut C30 Yu, X. B.;H. S. Yun;Y. M. Koo