Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Cloning of a Paenibacillus sp. Endo-${\circ}$-1,4-Glucanase Gene and Its Coexpression with the Endomyces fibuliger ${\circ}$-Glucosidase Gene in Saccharomyces cerevisiae

  • KIM, HYUNJIN (Division of Biological Sciences, College of Natural Sciences, Pusan National University) ;
  • JI-YOUNG YANG (Division of Food Science and Biotechnology, Pukyung National University) ;
  • HYEON-GYU LEE (Department of Food and Nutiriotion, Hanyang University) ;
  • JAEHO, CHA (Division of Biological Sciences, College of Natural Sciences, Pusan National University)
  • Published : 2001.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

A gene, Egl, from Paenibacillus sp. KCTC 8848P encoding endo-${\circ}$-1,4-glucanase was cloned and expressed in Escherichia coli. It consisted of an open reading frame of 1,191 bp for a protein that consisted of 397 amino acids with a molecular weight of 44,539 Da. The deduced amino acid sequence of the endo-${\circ}$-1,4-glucanase gene had a 94% similarity to the endo-$\beta$-1,4-glucanase of Bacillus polymyxa. The Egl gene was also expressed in Saccharomyces cerevisiae secreting Endomyces fibuliger $\beta$-glucosidase (BGL1) under the control of the alcohol dehydrogenase (ADC1) gene promoter, S. cerevisiae transformant producing both endo-${\circ}$-1,4-glucanase and ${\circ}$-glucosidase grew on carboxymethyl cellulose as the sole carbon source.

Keywords

References

  1. J. Bacteriol. v.172 Molecular cloning, expression and characterization of endo-β-1,4-glucanase gene from Bacillus polymyxa and Bacilluscirculans Baird, S. D.;D. A. Johnson;V. L. Seligy
  2. J. Microbiol. Biotechnol. v.1 Purification and charcaterization of β-glucosidase from Penicillium verruculosum Chun, S.-B.;D.-H. Kim;K.-H. Kim;K.-C. Chung
  3. Biotechnol. Genet. Eng.Rev. v.3 The properties of fungal and bacterial cellulases with comment on the production and application Couhlan, M. P.
  4. Microbiol. Rev. v.55 Domains in microbial β-1,4-glucanases:Sequence conservation, function, and enzyme families Gilkes, N. R.;B. Henrissat;D. G. Kildrun;R.C. Miller;R.A.J. Warren
  5. Enzyme Microbial Technol. v.6 Kinetics and mathematical model of hydrolysis and transglycosylation catalysed by cellulose Gusakov, a. V.;A. P. Sinitsyn;G. H. Goldsteins;A. A. Klyosov
  6. J. Microbiol. biotechnol. v.4 Secretion of Bacillus endoglucanase in Saccharomyces cerevisiae by its own signal sequence Han, Y.-J.;D.-O. Kang;S.-C. Lee;B.-Y. Kim;H.-H. Suh;J.-M. Kim;T.-I. Mheen;J.-S. Ahn
  7. Biochem. J. v.293 New families in the classification of glycosyl hydrolases based on amino acid sequence similarities Henrissat, B.;A. Bairoch
  8. Yeast v.2 Yeast/E. coli shuttle vectors with multiple unique restriction sites Hill, J.E.;A. M. Myers;T. J. Koemer;A. Tzaglolff
  9. Nucl. Acids Res. v.19 DMSO-enhanced whole cell yeast transformation Hill, J.;K. A. lan;G. Donald;D. E. Griffiths
  10. Biotechnol. Lett. v.22 Cloning and expression in Saccharomyces cerevisiae of a β-amylase gene from the oomycete Saprolegnia ferax Kim, H.-O.;J.-N. Park;H.-J. Sohn;D.-J. Shin;C. Choi;S.-Y. Im;H. B. Lee;S.-B. Chun;S. Bai
  11. J. Microbiol. Biotechnol. v.4 Construction of transformed yeast strain secreting both α-amylase and glucoamylase for direct starch-fermenation Kim, K.;J.-W. Lee
  12. Appl. Biochem. Biothnol. v.59 The construction of a stable starch-fermenting yeast strain using genetic engineering and rare-mating Kim, T.-G.;K. Kim
  13. Kor. J. Appl. Microbiol. Biotechnol. v.23 Simultaneous expression of Pseudomonas sp. endo-β-1,4-glucanase and β-glucosidase gene in Excherichia coli and Saccharomyces cerevisiae Kim, Y.-W.;S.-S. Chun;Y.-C. Chung;N.-K. sung
  14. Biotechnol. Lett. v.22 Cloning, expression and nucleotide sequences of two xylanase genes from Paenibacillus sp. Lee, H.-J.;D.-J. Shin;N.-C. Cho;H.-O. Kim;S.-Y. Shin;S.-Y. Im;H. B. Lee;S.-B. Chun;S. Bai
  15. Enzyme Microbial Technol. v.9 Use of Bacilli for overproduction of exocellular endo-β-1,4-glucanase encoded by clonedd gene Lee, D. S.;M. Y. Pack
  16. J. Bacteriol. v.175 Nucleotide sequence of the CeIG gene of Clostridium thermocellum and Characterization of its product, endoglucanase CelG Lemaire, M.;P. Beguin
  17. Nucl. Acids Res. v.14 Structure of a Bacillus subtilis endo-β-1,4-glucanase gene MacKay, R. M.;A. Lo;G. Willick;M. Zuker;S. Baird;M. Dove;F. Moranelli;V. Seligy
  18. Appl. Environ. Microbiol. v.54 Nucleotide sequences of Saccharomycopsis fibuligera genes for extraqcellular β-glucosidases as expressed in Saccharomyces cerevisiae machida, M.;I. Ohtsuki;S. Fukui;I. Yamashita
  19. Biosci. Biotech. Biochem. v.61 A gene encoding endo-β-1,4-glucanase from Bacillus sp. 22-28 Miyatake, M.;K. Imada
  20. Nucleic Acids Res. v.8 Rapid isolation of high molecular weight plant DNA Murray, M. G.;W. F. Thompson
  21. Appl. Environ. Microbiol. v.64 Molecular characterization and heterologous expression of the gene encoding a low molecular-mass endoglucanase from Trichoderma reesei QM9414 Okada, H.;K. Tada;T. Sekiya;K. Yokoyama;A. Takahashi;H. Kumagai;Y. Morikawa
  22. J. Microbiol. Biotechnol. v.9 Expression of Schwanniomyces occidentalis α-amylase gene in Saccharomyces cerevisiae var. diastaticus Park, J.-N.;D.-J. Shin;H.-O. Kim;D.-H. Kim;H. B. Lee;S.-B. Chun;S. Bai
  23. Mol. Gen. Genet. v.195 Cloning and expression of the structural gene for β-glucosidase of Kluyveromyces fragilis in Escherichia coli and Saccharomyces cerevisiae Raynal, A.;M. Guerineau
  24. Gene v.59 Efficient secretion of Bacillus anyloliquefaciens α-amylase by its own signal peptide from Saccharomyces cerevisiae Host Ruohonen, L.;P. Hackmann;P. Lehtovaara
  25. Molecular Cloning A Laboratory Manual (3rd ed.) Sambrook, J.;D.W. Russell
  26. Methods in Yeast Genetics, Laboratory Course Manual Sherman, F.;G. Fink;J. B. Hicks
  27. J. Antibiot. v.46 Biological properties of aureobasidin A, a cyclic depsipeptide antifungal antibiotic Takesako, K.;H. Kuroda;T. Inoue;F. Haruna;Y. Yoshikawa;I. Kato;K. Uchida;T. Hirataini;H. Yamaguchi
  28. Appl. Environ. Microbiol. v.43 Use of Congo redpolysaccharide interactions in enumeration and characterization of cellulolytic bacteria from bovine rumen Teather, R.M.;P.J. Wood
  29. Curr. Genet. v.30 Co-expression of a phanerochaete chrysosporium cellobiohydrolase gene and a Btyrovibrio fibrisoivens endo-β-1,4-glucanase gene in Saccharomyces cerevisiae Van Rensburg, P.;W. H. Van Zyl;I. S. pretorius
  30. Yeast v.14 Engineering yeast for efficient cellulose degradation Van Rensburg, P.;W. H. Van Zyl;I.S. Pretorius
  31. Nucl. Acids Res. v.14 A new method for predicting signal sequence cleavage sites von Heijne, G.
  32. J. Gen. Microbiol. v.128 Purification and some properties of the extracellular β-D-glucosidase of the cellulolytic fungus Trichoderma Koningii Wood, T. M.;S. I. McCrae
  33. Biotechnol. Lett. v.14 Simultaneous production of endoglucanase and β-glucosidase using synthetic two cistron genes Yoo, Y.D.;M.Y. Pack