한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제30권4호
  • /
  • Pages.600-604
  • /
  • 2001
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  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

감마선 조사된 새우의 검지를 위한 면역분석법의 적용

Application of Immunoassay for the Detection of Gamma-Irradiated Shrimp

  • 이주운 (한국원자력연구소 방사선식품생명공학기술개발팀) ;
  • 육홍선 (한국원자력연구소 방사선식품생명공학기술개발팀) ;
  • 조경환 (한국원자력연구소 방사선식품생명공학기술개발팀) ;
  • 차보숙 (수원여자대학 식품과학부) ;
  • 변명우 (한국원자력연구소 방사선식품생명공학기술개발팀)
  • 발행 : 2001.08.01
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초록

감마선 조사된 새우의 신속한 판별을 위한 방법으로 갈색새우의 TPM을 항원으로 하여 개별적으로 생산된 M-IgG와 P-IgG를 이용한 Sandwich ELISA를 분석법으로 확립하였다. M-IgG를 항원 포획을 이한 coating 항체로 사용하고, P-IgG를 포획된 TPM에 대한 반응항체로 사용하였을 때, 12.5에서 50$\mu\textrm{g}$/mL의 농도 범위에서 TPM을 정량할 수 있었다. 감마선 조사된 새우의 TPM 농도는 선량에 의존하며 감소하였고, 감마선 조사와 가열 또는 냉동 등의 병용 처리에서도 선량에 의존하며 감소하였다. 이 결과는 면역분석기법의 하나인 Sandwich ELISA가 감마선 조사된 새우의 검지법으로 이용될 수 있다는 가능성을 나타내었다.

Immunoassay was used to study the detection method of irradiated shrimp. Sandwich ELISA was formatted with monoclonal antibody (Ab) (M-IgG) and polyclonal Abs (P-IgG) and polyclonal Abs (P-IgG) individually produced against brown shrimp tropomyosin (TPM) as an antigen. When M-IgG was used as a coating Ab to capture TPM, and P-IgG were used as reaction Ab against captured TPM could be detected in the range of 12.5 to 50 $\mu\textrm{g}$/mL. Detected concentrations of TPM from irradiated shrimp decreased dose-dependently, and the concentration of Ag by combination of irradiation with heating or freezing treatments also decreased. This results suggests the possibility for Sandwich ELISA, one of immunological analyses, to be applied for detecting irradiated shrimp.

키워드

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