Quantitative Analysis of Dietary Fibers from Perilla frutescens Seeds and Antimutagenic Effect of Its Extracts

들깨의 식이 섬유소 함량분석과 들깨 추출물의 항돌연변이 효과

  • 박동숙 (부산대학교 식품영양학과 및 김치연구소) ;
  • 이경임 (양산대학 호텔조리과) ;
  • 박건영 (부산대학교 식품영양학과 및 김치연구소)
  • Published : 2001.10.01

Abstract

In this study, the levels of insoluble dietary fiber(IDF) and soluble dietary fiber (SDF) in Perilla frutescens seed were quantified and antimutageinc effects of perilla seeds extracts (method extract, hexane extract, methanol soluble fraction and dietary fiber)was carried out IDF and SDF values of perilla seeds were 16.1% and 1.1% , respectively, with 17.2 of total fiber content. Among the solvent extracts of perilla seeds, methanol extract and methanol soluble fraction (MSF) effectively inhibited the mutagenicity induced by aflatoxin B$_{1}$(AFB$_{1}$)in Salmonella typhimurium TA100, Methanol extract of perilla seeds showed 91% inhibition against AFB$_{1}$ mutagen under the 2.5 mg/assay concentration, and MSF inhibited the mutagenicity of 87% by adding 1.25,g/assay. However, perilla seed extracts showed low inhibition rate on the mutagenicity induced by N-methyl-N-nitro-N-nitosoguanidine(MNNG). And also, SDF and hexane extracts from perilla seeds did not show the antimutagenic effects against AFB$_{1}$ and MNNG. On the hand, IDF extracted from perilla seeds inhibited 21% of mutagenicity induced Trp-P-2 due to the carcinogen binding effect.

본 연구에서는 들깨의 불용성 및 수용성 식이 섬유소의 함량을 측정하였으며 들깨의 methanol 추출물, hexane 추출물, MSF 및 식이 섬유소를 추출하여 항돌연변이 효과를 관찰하였다. 들깨의 총 식이 섬유소의 함량은 신선물인 경우 17.2%였고 건조물인 경우 18.2%였으며 이 가운데 94%가 불용성식이 섬유소였다. 들깨의 용매 추출물 중에서 methanol 추출물과 MSF는 AFB$_{1}$에 의해 유발된 돌연변이를 상당히 억제시켰으며 methanol 추출물과 MSF를 2.5mg/assay 첨가군에서 각각 91%와 87%의 억제효과를 나타내었다. 그러나 같은 농도에서 SDF의 첨가군은 억제효과가 나타나지 않았고 hexane 추출물은 20%의 억제효과를 나타내었다. 한편 직접 돌연변이원인 MNNG를 돌연변이원으로 사용한 경우에 항돌연변이 효과는 크게 나타나지 않았다. 즉 SDF를 1.25mg/assay 첨가한 군은 17%, MSF 2.5mg/assay 첨가군은 27%의 저해율을 보였을 뿐 methanol과 hexane 추출물 첨가군은 억제효과를 나타내지 않았다. 따라서 들깨의 용매 추출물은 간접 돌연변이원에 의한 돌연변이성을 억제시키는 것을 알수 있었다. 또한 불용성 식이 섬유소의 경우 Trp-P-2에 의해 유발된 돌연변이를 다소(13~18%)억제 하는것을 알 수 있다.

Keywords

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