Comparison of Physiological Activity in Different Parts of Dolsan Leaf Mustard

돌산 갓의 부위별 생리활성 작용의 비교

Physiological activity in different parts of Dolsan leaf mustard (DLM) was investigated. To determine the physiological activity, DLM was crushed filtered centrifuged and then the supernatant was used as a sample. of 1%, 3% and 6%. By adding of improved root juice(20th day) with the concentration of 6% the cytotoxicity against HepG2 was the highest about 78.2%. And antioxidative effects in different parts of DLM was measured by using the DPPH method. Antioxidative effects was higher in all leaves than other parts. In particular antioxidative effects was the highest in leaves of traditional DLM at the 20th day of growth about 80.4%. In leaves of improved DLM at the 60th growth angiotensin-I converting enzyme inhibitory effect was the highest about 94.0%. Consequently there was not significant difference of physiological activity between improved and traditional DLM. However the cytotoxicity against HepG2 was the highest in roots of DLM. And the antioxidative and the ACE inhibitory effect in leaves of DLM were higher than those of other parts.

성장 시기에 따른 돌산 갓의 부위별 즙액 첨가에 의한 생리기능성을 연구하였다. 생리기증성을 평가하기 위하여 돌산갓을 분쇄하여 여과하고 원심 분리하여 얻은 상등액을 여과하여 시료로 사용하였다. 세포독성 평가를 위한 돌산 갓 즙액의 농도는 1%, 3%, 6% 로 조제하여 간암세포에 첨가하였다. 세포독성은 갓 즙액 6% 첨가 시에 성장기간이 20일인갓의 개량종 뿌리에서 78.2%로 가장 높게 나타났다. DPPH 방법에 의한 항산화 활성 평가에서는 20일 자란 재래종 잎이 80.4%로서 가장 높게 나타났다. ACE 저해 활성으로는 60일 자란갓에서는 개량종 잎이 94.0%로서 가장 ACE 저해율이 높은 것으로 나타났다. 따라서 돌산 갓의 부위별 첨가에 의한 생리기능성 평가에서 재래종과 개량종 사이에 큰차이를 발견할 수는 없었으나 부위별로 항암성은 뿌리에서 항산화성과 ACE 저해율은 잎에서 비교적 활성이 높게 나타났다.