Antioxidative Activity of Solvent Fraction and Isolation of ANtioxidative Compound from Chestnut Husk

밤 귀피의 용매분획별 항산화 활성과 항산화 물질의 분리

  • 권은정 (경북대학교 식품공학과) ;
  • 김영찬 (경북대학교 식품공학과) ;
  • 권미선 (경북대학교 식품공학과) ;
  • 김창섭 (경북대학교 농업개발대학원) ;
  • 강우원 (상주대학교 식품영양학과)
  • Published : 2001.08.01

Abstract

To enhance the utilization of chestnut husk discarded in the processing company antioxidative activities and compounds were investigated. Antioxidative activities of solvent fractions from chestnut husk were examined by benzoic acid hydroxylation method ferric thiocyanate method and DPPH test. Ethyl acetate fraction showed strong antioxidative activities comparable to BHA. Active compounds were isolated and purified from ethyl acetate fraction by Sephadex LH-20 column chromatography and preparative HPLC. A major active compound gallic acid was identified by $^{1}$H and $^{13}$ C-NMR. The phenolic acid contents was analyzed by GC and ellagic acid (172.22 mg%) and gallic acid (107.39 mg%) were major phenolic acid of chestnut husk.

밤 가공공장에서 폐기되고 있는 밤의 귀피를 항산화 기능성 자원으로 활용하고자 밤 귀치의 methanol 추출물 및 용매획분을 benzoic acid 수산화법으로 hydroxyl radical 소거능과 ferric thiocyanate법으로 과산화물생성 억제능을 시험하였다. 대부분의 획분에서 항산화성을 보였으며 특히 ethyl acetate 획분에서 BHA나 $\alpha$-tocopherol과 거의 유사한 rkdgkshydroxyl radical 소거능과 지질과산화물생성 억제능을 보였다. Ethyl acetate 획분으로부터 항산화성 물질을 분리하기 위하여 Sephadex LH-20 column chromatography 및 prep HPLC를 행하여 단일 물질로 정제하였다. 분리 정제된 물질을 $^{1}$H-NMR과 $^{13}$C-NMR로 구조 분석한 결과 gallic acid로 동정하였다. 페놀산을 GC로 분석한 결과 밤 귀피의 주요 페놀산으로 ellagic acid(172.22 mg%) 와 gallic acid(107.39 mg%)가 확인되었다.

Keywords

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