누에고치 산 가수분해물의 단백질의 질적 평가와 고콜레스테롤, 고지질, 고당질식이 흰쥐의 혈장지질에 미치는 영향

Protein Quality Evaluation and Effect of Plasma Lipid Contents of Acid Hydrolysates of Cocoon inn Rats Fed by High Cholesterol, High Triglyceride and High Sucrose Diet

  • 황은희 (원광대학교 식품영양학과) ;
  • 강병기 (원광대학교 한의과대학 내과학교실) ;
  • 김복량 (원광대학교 의과대학 생화학교실) ;
  • 이형자 (원광대학교 식품영양학과)
  • 발행 : 2001.10.01

초록

누에고치를 2N HCl 용액으로 11$0^{\circ}C$, 48시간 가수분해한후 중화하여 냉동건조하여 얻는 누에고치 산가수분해물의 아미노산 조성은 glycin이 43.25%, alanine이 34.39%, serine 10.05%, valine이 2.44%였고 필수 아미노산은 7.12% 차지하였다. 누에고치 산가수분해물의 사료이용효율은 카제인과 같았다. 1~5% 실크분말의 첨가에 의한 간의 무게, 간효소활성(GOT, GPT), 혈청알부민, 혈청 총단백질의 함량은 영향이 없어 단백질의 질이 낮은 것으로 평가되었다. 흰주에 고콜레스테롤, 고지질, 고설탕사료를 각각 섭취시키면서 5% 누에고치 산가수분해물을 첨가하였을 때 혈장 지질농도에 미치는 영향은 중성지질의 감소, 인지질과 HDL의 증가 등 혈액지질조정에 바람직한 결과를 보였으나, LDL과 총콜레스테롤을 높이는 좋지 못한 효과도 나타냈다. 그러므로 누에고치 산가수분해물은 단백질 급원 식품으로보다는 이를 이용한 펩타이드의 생리기능 또는 식용 이외의 화장품재료, 의약용재료, 포장제 등과 기타 물리 화학적 특성을 기초로 한 다양한 범위의 응용이 더욱 적합하다고 생각되며, 혈액지질 개선효과에 대해서는 심층적인 연구가 필요하다.

Acid hydrolysates of cocoon was gained by acid hydrolysis of 2 N HCl, 11$0^{\circ}C$, 48 hours, neutralization and desalting from the cocoon. The amino acid compositions of acid hydrolysates of cocoon were glycine 43.25%, alanine 34.39%, serine 10.05% and valine 2.44%. The contents of essential amino acid was 10.05%. Food efficiency ratio of acid hydrolysates of cocoon group was equal to the reference protein, casein. Liver weight, GOT, GPT activity, serum albumin and serum total protein level of rats were not significantly different among the experimental groups. Therefore, the protein acid hydrolysates of cocoon is not of high quality. When the rat fed with high cholesterol, high lipid, and high sucrose diet was administered with 5% acid hydrolysates of cocoon, its plasma lipids concentration of acid hydrolysates of cocoon was favorably affected: its triglyceride was decreased, and the level of phospholipid and HDL cholesterol were increased. There was also an unfavorable effect: the levels of LDL cholesterol and total cholesterol went up. Therefore, the acid hydrolysates of cocoon is not a good protein food source, but is can be used a cosmetic, medical, or packing material. Further research will reveal how it will affect or improve plasma lipid.

키워드

참고문헌

  1. Kor. J. Food Ind. Nutr. v.4 Market tendency and development of physiological active peptide Nam, H. S.
  2. Kor. J. Food Sci. Ind. v.25 Application and development of protein sources Lee, C. H.
  3. Protein: Biochemistry and Application in Encyclopedia of Food Science and Technology v.3 Rupnow, J.Ⅱ.;Hui, Y. H.(ed.)
  4. J. Seric. Sci. Jpn. v.63 Production of silk powder and properties Lu, X.;Akiyama, D.;Hirabayashi, K.
  5. J. Seric. Sci. Jpn. v.60 Production of souble fibroin powder by hydrolysis with hydrochloric acid and physical properties Chen, K.;Takanono, R.;Hirabayashi, K.
  6. 실크과학 남중희;신봉섭
  7. New Food Industry v.31 絹っイづロイシの食品素材としての活用 平林 潔;陳 開利
  8. J. Seric. Sci. Jpn. v.60 The digestion of fibroin by rat Chen, K.;Iura, K.;Aizawa, R.;Hirabayashi, K.
  9. 絲科學の 技術 v.33 絹の 多面的 移用の 可能性 問 和夫
  10. Seni Gakkashi(纖維と工業) v.45 絹の ゲル化の 應用 (食品) 平林 潔:渡邊 誠;鈐木 誠
  11. 絲科學の 技術 v.32 絹利用の 多目的 展開 平林 潔;陳 開利
  12. Kor. J. Monthly Seric. v.16 Method and preparation of silk powder Lee, Y. W.
  13. New Food Industry v.5 絹を 食へる 平林 潔;平岩 陽
  14. Argic. Bio. Chem. v.49 Effect of sulfur containning amino acid and glycine on plasma cholesterol level in rats fed on a high cholesterol diet Sugiyama, K.;Kushima, Y.;Muramatsu, K.
  15. Kor. J. Food Industry v.136 Edible usage technology of the coconon on functional food Lee, S. G.
  16. Kor. J. Monthly Seric. v.20 Health promotion effect and ediblelize technique of the cocoon Lee, S. G.
  17. J. Agric. Sci. v.37 A study of pharmachological effect of silk fibroin Nam, J. K.;Oh, Y. S.
  18. 고급영양학 이기열;이양자
  19. Horm. Metab. Res. v.30 A High sucrose diet ulters the lipid composition and fluidity of liver sinusoidal membranes Podolin, D. A.;Sutherland, E.;Iwahashi, M.;Simon, F. R.;Pagliassotti, M. J.
  20. Am J. Physiol. Endocrinol. Metab. v.280 A high sucrose diet increases gluconeogenic capacity in isolated periportal and perivenous rat hepatocytes Bizeau, M. E.;Thresher, J. S.;Pagliassotti, M. J.
  21. Endocr. Regul. v.30 Regulation on gene expression and activity of malic enzyme in liver of hereditary hypertriglyceridemic (hHTG) insulin resistant rat: effect of deitary sucrose and amrine fish oil Sebokova, E.;Klimes I. I.;Knopp, J.;Gasperikova, D.;Bohov, P.;Langer, P.;Clandinin, M. T.
  22. 영양상태판정 김화영;강병희;조미숙
  23. Kor. J. Nutr. v.27 Plasma lipids and fecal excretion of lipids in rats fed a high fat diet, a high cholesterol diet or a low fat/high sucrose diet Park, O. J.
  24. Kor. J. Nutr. v.27 Effect of the soy protein level on plasma glucose lipids, and hormons in Streptozotosin-diabetic rats Choi, M. J.;Han, Y. J.
  25. Kor. J. Nutr. v.26 Effects of the protein level on plasma glucose, lipids and hormones in Streptozotosin-diabetic rats Han, Y. J.;Choi, M. J.
  26. Kor. J. Nutr. v.26 Effect of diet protein and magnesium level on serum lipid contents and enzyme activities in rats Jung, B. M.;Sung, C. J.
  27. Kor. Society of Nutrition, 1997 Spring Symphosium Effect of blood glucose and serum lipid level on diabetic paitient by the silkworm powder Cho, M. R.;Cho, Y. W.;Jung, S. H.;Rhu, J. H.
  28. Clin. Sci. v.27 The influence of dietary carbohydrate on the lipid pattern in serum and in adipose tissue Macdonald, I.;Braithwaite, D. M.
  29. J. Am. Clin. Nutr. v.5 Deitary protein effects on cholesterol and lipoprotein concentrations: a review Forsythe, W. A.;Green, M. S.;Anderson, J. B.
  30. Kor. J. Seric Sci. v.39 Hydrolysis of silk fibroin on alkali conditions Kim, N. J.;Bae, D. G.
  31. Yakhak Hoeji v.39 Blood glocose-lowering effects of Mori Folium Lee, J. S.;Choi, M. H.;Chung, S. H.
  32. Kor. J. Diabets v.18 Survey of folk medicine survey of adult diabetic paitients by questionare Nam, M. S.;Kim, K.R.;Cho, J. H.;Lee, K. M.;Park, H. Y;Lee, E. J.; Lim, S. G.;Lee. H. C.;Huh, G. B.
  33. Kor. J. Food Sci. Technol. v.31 Anti-hyperglycemic effect of Cortex Mori radicals in db/db mice Kim, Y. Y.;Choue, R. W.;Chung, S. H.;Koo, S. J.
  34. Planta Media. v.159 Isolation and hypoglycemic activity of Moran A. a glycoprotein of morus alban Root Barks Hikino, H.;Mizuno, T.;Oshima, Y.;Konno, C.