Characterization and Condition of Silver Accumulation Bacteria in Groundwater

지하수에서 분리한 은(銀) 축적균주에 대한 축적조건 및 특성

  • 배진희 (한양대학교 생명과학과) ;
  • 민병례 (상명대학교 생물학과) ;
  • 한명수 (한양대학교 생명과학과, 물환경생태복원연구실) ;
  • 최영길 (한양대학교 생명과학과, 물환경생태복원연구실)
  • Published : 2001.12.01

Abstract

The strain which accumulate the silver in cell were isolated and characterized. And condition of accumulation of heavy metal was examined closely to investigate optimal condition of accumulation. Pseudomonas fluorescens and Bacillus cereus were Isolated from groundwater as the strain of silver accumulating bacteria. These strains did not grow in the medium at silver over the concentration 20 ppm. The largest accumulations of silver in the culture of Pseudomonas fluorescens and Bacillus cereus occurred within 24 hours. The amount of silver accumulation in Pseudomonas fluorescens and Bacillus cereus were 1.9 mg/g cell and 1.65 mg/g cell, repletively. In protein patterns of cell after the treatment of silver, three reducible proteins (126 KDa, 89 KDa, 25 KDa) in Bacillus cereus and one new protein (34 KDa) in Pseudomonas fluorescens were detected by SDS-PAGE.

본 연구에서는 중금속, 특히 은(銀)을 축적하는 세균을 지하수로부터 분리, 동정하고, 분리 균주의 특성 및 중금속 축적조건을 규명하여 최대축적이 일어날 수 있는 조건을 검토하였다. 서울시 지하수 10개 정점으로부터 분리된 은(銀) 축적 세균 중 가장 축적능이 뛰어난 두 균주를 선별하였으며 이들은 Pseudomonas fluorescens와 Bacillus cereus로 동정되었다. 본 분리 균주는 은(銀)의 농도가 5 ppm, 10 ppm, 20 ppm으로 높아질수록 생장 저해를 심하게 받았다. pH가 낮을수록 세포의 성장이 저하되기는 하였지만 세포 당 은(銀) 축적율은 오히려 증가하였으며 24시간 이내에 최대축적이 이루어졌다. 이때 은(銀) 축적량은 Pseudomonas fluorescens의 경우 약 1.9 $mg/g{\cdot}cell$ 이었으며 Bacillus cereus는 약 1.65 $mg/g{\cdot}cell$ 이었다. 은(銀) 처리시, Bacillus cereus에서 126 KDa, 89 KDa, 25 KDa 등 3개의 단백질이 특이적으로 증가하였으며, Pseudomonas fluorescens에서는 101 KDa, 68 KDa, 27 KDa 크기의 단백질이 감소하였으나 34 KDa 크기의 새로운 단백질이 유도됨을 확인하였다.

Keywords

References

  1. J. Biochem. v.90 Cadmium-binding peptide induced in fission yeast Schizosaccharomyces pombe Akira, M.;C. Wada;Y. Hayashi.
  2. Appl. Environ. Microbiol. v.44 Adaptation to cadmium by Klebsiella aerogenes growing in continuous culture proceeds mainly via formation of cadmium sulfide Aiking, H.;K. Kok;H. van Heerikhuizen;J.van't Riet
  3. J. Bacteriol. v.141 Site of metal doposition in the cell wall of bacillus subtilis Beveridge, T.J.;R.G.E. Murray
  4. Metal Based Drugs v.1 no.#5-6 Antibacterial silver Clement, J.C.;P.S. Jarrett.
  5. J. Biol. Chem. v.261 Teast metallothionein function in metal ion detoxification Ecker, D.J.;T.R. Butt;E.J. Sternberg;M. P. Neeper;C. Debouck;J.A. Gorman;S.T. Crooke
  6. Experientia v.46 Heavy metal accumulation by bacteria and other microorganisms Gadd, G.M.
  7. Ann. Rev. Biochem. v.55 Metallothionein Hamer, D.H.
  8. Science v.225 Cadmium-resistant Pseudomonas putida synthesizies novel cadmium proteins Higham, D.P.;P.J. Sadler
  9. Kor. J. Appl. Mocrobiol. Bioeng. v.4 Isolation of cadmium-tolerant bacteria and accumulation of cadmium into the bacteria cell Kim, Y.B.;S.R. Lee
  10. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T. Laemmli, V.K.
  11. The development and functions of silver in water purification and disease control v.36 Richard L.D.;S.F. Etris
  12. Appl. Environ. Mocrobiol. v.41 Microbial cells as biosorbents for heavy metals : accumulation of uranium by Saccharomyces serevisiae and Pseudomonas aeruginosa Standberg, G.W.;S.E. Shumate;J.R. Parrott
  13. Comprehensive biotechnology v.4 Shumate S.E.;G.W. Strandberg;Robinsion C.W.(ed.);Howell J.A.(ed.)
  14. J. Indust. Microbiol. v.14 A rapid screening method for the isolation of metal-accumulating microorganisms Pumpel, T.;B. Pernfuβ;B. Pigher;L. Diels;Schinner