Variation of the Intergenic Spacer (IGS) Region of Ribosomal DNA among Fusarium oxysporum formae Speciales

  • 발행 : 2001.12.01

초록

Variation within the intergenic spacer(IGS) of the ribosomal DNA gene for twenty-two strains of E. oxysporum and its formae speciales was examined by PCR, couped with RELP analysis. The length of the amplified IGS region was about 2.6 kb in all strains except F.oxysporum f. sp. cucumer-inum from Korea and F. oxysporum f. sp. niveum. Those two strains were 2.5 kb long. Restriction digestion of IGS-RELP regions by Eco RI, NruI, HincII, SAlI, SmaI, BalIi, HindIII, XhoI and KpnI gave rise to nine IGS hapoltypes among all strains. Cluster analysis based on the presence of absence of comigrating restriction reagments show the two groups based on 44% genetic similarity. These results demonstrated that analysis of IGS showed some difference within and between F. oxysporum formae speciales.

키워드

참고문헌

  1. Appl. Environ. Microbiol. Genetic diversity of Fusarium oxysporum strains from common bean fields in Spain Alves-Santos F.M.;E.P. Benito;A.P. Eslava;J.M. Diazminguez
  2. Experimen. Mycol. v.19 Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA Appel D.J.;T.R. Gordon
  3. MPMI v.9 Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergeinc spacer region of the ribosomal DNA Appel D.J.;T.R.;Gordon
  4. Mycologia v.88 Armillaria species of the Olympic peninsula of Washingron state, including conformation of North American Biological species ⅩⅠ. Banik M.T.;Volk T.J.;Bursall H.H. Jr.
  5. Plant Mol. Biol. v.33 Analysis of the ribosomal RNA gene repeat from the moss Funaria hygrometrica Capesins I.
  6. Plant Pathol. v.49 Variation in Fusarium gramineaum isolates from Nepal associated with their host of origin Carter J.P.;H.N. Rezanoor;A.E. Desjardins;P. Nicolson
  7. Ecology and Population Biology v.87 Populations of nonpathogenic Fusarium oxysporum associated with roots of four plant species compared to soilbome populations Edel V.;Steinbeg C.;N. Gautheron;C. Alabouvette
  8. Appl. Environ. Microbiol. v.65 The nuclear ribosomal DNA intergenic spaces as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on Pinus roof systems Guidot A.;Lumini E.;Debaud J.C.;R. Marmeisse
  9. Mycologia v.87 A PCR-based identification method for species of Armilaria Harrington T.C.;Wingfield B.D.
  10. Q. Rev. Biol. v.66 Ribosomal DNA: molecular evolution and phylogenetic inference Hills D.M.;Dixon M.T.
  11. Phytopathology v.83 Mitochondrial DNA (mtDNA)-Relatedness among formae speciales of Fusarium oxysporum in the Cucurbitaceae Kim D.H.;R.D. Martyn;C.W. Magill
  12. Fusarium wilt of Banana Molecular genetics of plant pathogenic Fusarium oxysporum Kistler H.C.;E.A. Momol;R.C. Ploetz(ed.)
  13. Phytopathology v.81 Repetitive genomic sequences for determining relatedness among strains of Fusarium oxysporum Kistler H.C.;E.A. Momol;U. Benny
  14. Phytopathology v.87 Genetic diversity in the plant-pathogenic fungus Fusarium oxysporum Kistler H.C.
  15. Fusarium-P.E. Nelson Memorial Symposium Evolution of host specificity in Fusarium oxysporum Kistler H.C.B.A. Summerell(ed.);J.F. Leslie(ed);D. Backhouse(ed.);W.L. Bryden(ed.);L.W. Burgess(eds.)
  16. J. Microbiol. v.38 PCR-RFLP and sequence analysis of the rDNA ITS region in the Fusarium spp. Lee Young-Mi;Y.K. Choi;B.R. Min
  17. J. Microbiol. v.36 Electrophoretic karyotypes of Fusarium oxysporum formae speciales Min B.R.;K.A. Kim;Y.K. Choi
  18. FEMS Microbiol. Letters v.108 PCR amplification of the 3' external transcribed and intergenic spacers of the ribosomal DNA repeat unit in three species of Sacchromyces Morina F.I.;S.C. Jong;J.L. Haffman
  19. Appl. Environ. Microbiol. v.60 Characterization of the formae speciales of Fusarium oxysporum causing wilts of Cucurbits by DNA fingerprinting with nuclear repetitive DNA sequences Namiki F.;T. Shiomi;T. Kayamura;T. Tsuge
  20. Gene v.249 Comparative analysis of intra-individual and inter-species DNA sequence variation in Salmonid ribosomal DNA cistron Reed K.M.;J.D. Hackett;R.B. Philips
  21. Ann. J. Bot. v.27 The species concept in Fusarium Snyder W.C.;H.N. Hansen
  22. Curr. Genet. v.30 Meiotic segregation and recombination of the intergenic spacer of the ribosomal DNA in the ectomycorrhizal basidiomycete Laccaria bicolor Selosse M.A.;G. Costa;C.D. Battista;F.L. Tacon;F. Marfin
  23. Mycoscience v.39 Indentification of Armiliaria species from Hokkaido by analysis of the intergenic spacer (IGS) region of ribosomal DNA using PCR-RFLP Terashima K.;Kawashima Y.;J.Y. Cha;K. Miura
  24. Mycologia v.88 Armillaria nabsnona, a new species from western North America Volk T.J.;Burdsall H.H. Jr.;Banik M.T.
  25. PCR protocols Amplification and direct sequencing of fungal ribosomal genes from phylogenetics White T.J.;T.D. Bruns;S. Lee;J. Taylor;Innis M.A.(ed.);Gelfraud D.H.(ed.);Sninsky J.J.(ed.);White T.J.(eds.)