Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Production of Nucleocapsid Protein of Newcastle Disease Virus in Escherichia coli and its Assembly into Ring-and Nucleocapsid-like Particles

  • Kho, Chiew-Ling (Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies. Universiti Putra Malaysis) ;
  • Tan, Wen-Siang (Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies. Universiti Putra Malaysis) ;
  • Khatijah Yusoff (Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies. Universiti Putra Malaysis)
  • Published : 2001.12.01
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Abstract

The nucleocapsid(NP) protein of Newcastle disease virus (NDV) and its derivative (NP$\sub$cfus)containing the myc region and six histidine residues fused to its C-terminus were pcpressed aboundantly in Escherichia coli. The proteins were purified by sucrose gradient centrifugation. Both the NP and NP$\sub$cfus/ proteins self-assem- bled into ring-like particles stacked together to from nucleocapsid-like structure which are heterogeneous in length with a diameter of 20${\pm}$2 nm and central holow of 5${\pm}$1 nm. Only a very small amount of the monomers in the particles was linked by inter-molecular disulfide bonds. Fusion of the C-terminal end to 29 amino acids inclusive of the myc epitope and His tag did not impair ring assembly buy inhibited the formation of the long herringbone structures. Immunogold lableing of the particles with the anti-myc antibody showed that the C-terminus of the NP$\sub$cfus/ protein is exposed on the surface of these ring-like particles.

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