Isolation of Angiotensin Converting enzyme inhibitors from Ripe Cucurbita moschata Duch

  • Hyeyoung Jung (Department of Food Science & Technology, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science & Technology, Chungnam National University)
  • Published : 2001.12.01

Abstract

Angiotensin converting enzyme (ACE) inhibitor acts on the inhibition of ACE and causes a decrease in blood pressure. There have been several reports on screening of ACE inhibitors from natural food products and protein hydrolysates of various food sources. Ripe Cucurbita moschata Duch has been used as an oriental medicine in Korea. To isolate ACE inhibitors, crude water extracts of the edible portion of ripe Cucurbita moschata Duch were obtained after heating in water at 95$^{\circ}C$ for 2 h. Crude extracts were then filtered using PM-10 and YM-1 membranes. The membrane-filtered solution was loaded onto Sephadex G-15 column equlibrated with a phosphate buffer. Among the four major fractions of gel permeation chromatography, the second fraction had the highest inhibitory activity of 65%. Further purification of the fraction using reversed-phase HPLC with a $C_{18}$ column produced ACE inhibitors, which were identified as a mixture having molecular mass of 222 and 273 by Tandem mass spectrometry.

Keywords

References

  1. J. Kor. Soc. Food. Sci. Nutr. v.26 Chemical components in different parts of pumpkin Park, Y.;Cha, H.;Park, M.;Kang, Y.;Seog, H.
  2. Korean J. Food Sci. Technol. v.29 Chemical composition of ripened and green pumpkin Cho, K.S.
  3. J. Food Sci. Nutr. v.6 Effect of extraction on the anticomplementary activity of green and ripe Cucurbita moschata Duch Yang, J.;Kim, C.;Song, K.B.
  4. Trends Food Sci. Technol. v.4 Angiotensin-converting enzyme inhibitors derived from food proteins Ariyoshi, Y.
  5. Biosci. Biotech. Biochem. v.57 Inhibition of ACE by B. licheniformis alkaline protease hydrolyzates derived from sardine muscle Matusi, T.;Matsufuji, H.;Seki, E.;Osajima, K.;Nakashima, M.;Osajima, Y.
  6. J. Food Sci. Nutr. v.3 Partial purification of ACE inhibitory peptide isolated from supernatnat of bovine plasma treated by trichloroacetic acid Park, E.;Song, K.B.
  7. J. Food Sci. Nutr. v.4 Comparison between bovine hide and pigskin gelatins and preparation of gelatin hydrolysates Shin, S.;Song, K.B.
  8. J. Food Sci. Nutr. v.5 Partial purification of mussel adhesive protein from Mytilus edulis and preparation of mussel protein hydrolysates Sun, N.K.;Song, K.B.
  9. Agric. Chem. Biotechnol. v.44 Isolation of an angiotensin converting enzyme inhibitor from Oenanthe javanica Noh, H.;Song, K.B.
  10. Biochem. Pharmacol. v.20 Spectrophotometric assay and properties of the ACE of rabbit lung Cushman, D.W.;Cheung, H.S.