Antioxidative Activity of Mustard Leaf Kimchi Added Green Tea and Pumpkin Powder

녹차 및 늙은호박분말 첨가에 따른 갓김치의 항산화 효과

  • 박민정 (부산대학교 식품영양학과 및 김치연구소) ;
  • 전영수 (부산대학교 식품영양학과 및 김치연구소) ;
  • 한지숙 (부산대학교 식품영양학과 및 김치연구소)
  • Published : 2001.12.01

Abstract

This study was conducted to estimate the content of antioxidant components and investigate the antioxidative activity of mustard leaf kimchi added pumpkin powder (P) and green tea powder (G). Mustard leaf kimchi added different ratios of P and G were prepared and fermented for 30 days at 5$^{\circ}C$ (properly fermented time). The contents of chlorophyll, total phenol, carotenoids and ascorbic acid in mustard leaf kimchi samples were estimated. The 30 day fermented mustard leaf kimchi added 0.3% P and 0.2% G had relatively higher content of antioxidant components than the other kimchi samples. Based on this result, antioxidative activities of mustard leaf kimchi added 0.3% P and 0.2% G and control kimchi were investigated, that is, methanol extracts of the mustard leaf kimchi samples were prepared and investigated the antioxidative activities. The antioxidative activity of mustard leaf kimchi added 0.3% P and 0.2% G was significantly (p<0,05) higher than control kimchi, and that of the 30 day fermented mustard leaf kimchi at 5$^{\circ}C$ was also significantly higher (p<0.05) than the fresh mustard leaf kimchi. Therefore, it is suggested that antioxidative activity of the 30 day fermented mustard leaf kimchi added 0.3% P and 0.2% G at 5$^{\circ}C$ was the best compared to the other kimchi samples.

갓김치에 항산화 성분의 함량이 높은 부재료를 첨가하여 기능성이 증진된 갓김치를 개발하기 위하여 본 연구를 행하였다. 부재료로 선정된 녹차 및 늙은호박을 분말로하여 이들의 첨가비를 달리 한 갓김치를 제조한 후 chlorophyll, carotenoids, 총 페놀 및 비타민 C 함량을 담금 직후와 적숙기에 측정하여 부재료 첨가에 따른 갓김치의 항산화 성분 함량을 비교 연구하였으며 , 그 결과 늙은호박분말 0.3%와 녹차분말 0.2%을 첨가한 갓김치구가 높은 함량을 나타내었다. 이에 늙은호박분말 0.3%와 녹차분말 0.2%을 첨가한 갓김치구를 대조구과 함께 비교하면서 항산화성에 대한 실험을 행하였다 항산화성 실험은 갓김치를 담은 첫날과 적숙기에 행하였으며, 그 결과 거의 모든 실험에서 대조구보다 녹차 및 늙은호박을 첨가한 갓김치구가 항산화성을 나타내는 산화에 대한 저해율이 높게 나타났다. 담금 첫날 갓김치 구보다 적숙기의 갓김치구가 힝산화성이 높았는데, linoleic acid 자동산화에 대한 억제효과는 담금 첫날 대조구의 저해율 42.81%보다 늙은호박분말 0.3%와 녹차분말 0.2% 첨가 처리구가 46.95%로 더 높았으며, 적숙기에는 대조구는 58.63%, 처리구는 65.91% 으로 적숙기의 처리구가 다른 군에 비해 가장 높았다. Conjugated diene생성 억제효과는 적숙기의 처리구는 55.50%로 가장 높았고, LDL 산화 저해효과, TBA가 측정 결과도 적숙기의 녹차 및 늙은호박분말 첨가구가 가장 높은 저해율을 나타내었다. 그리고 DPPH 소거효과에서 담금 첫 날 대조구의 51.23%에 비해 적숙기의 처리구는 76.81%로 저해율이 아주 높게 측정되었는데 이는 부재료 첨가 갓김치구는 DPPH에 대한 강한 소거작용을 하는 것으로 보인다. 따라서 이상과 같은 실험 결과를 종합할 때 갓김치에 녹차 및 늙은호박분말을 첨가함으로써 첨가하지 않은 갓김치 대조구에 비해 항산화 관련 성분의 함량도 증가하였고, 또한 항산화성 실험 에서도 산화에 대한 저해율이 높게 나타났으며 그 중에서도 갓김치에 늙은호박분말 0.3%와 녹차분말 0.2% 첨가 갓김치구가 가장 우수하였고 특히 적숙기에 가장 높은 항산화성을 보이는 것으로 나타났다.

Keywords

References

  1. Agric. Biol. Chem. v.49 Inhibition of xanthine oxidase by flavoonoids IIo. M.;Moriyama. A.;Matsumoto. Y.;Takaki. N.;Fukumoto. M.
  2. Agric. Chem. Biotechnol v.39 Antioxidative activity of phenolic acids from Jindalrae flower(Rhododendron Mucronulatum Turzaninow) Chung. T.A.;Kim. M.A.;Jones. A.D.
  3. Science of kimchi Nutrient physiological assessment of kimchi Oh. Y.J.;Hwang. Y.J.;Claus. L.
  4. Free Rad. Res. Comm. v.2 Radical scavenging by flavonoid antioxidants Bros. W.;Saran. M.
  5. Food antioxidants. Hudson. B.J.F.
  6. J. Korean Soc. Food Sci. Nutr. v.26 Effect of kimchi ingredients to reactive oxygen species in skin cell cytotoxicity Ryu. S.H.;Jeon. Y.S.;Kwon. M.J.;Moon. J.W.;Lee. Y.S.;Moon. G.S.
  7. Korean J. Dietary Culture v.8 Contents of carotenoids and chlorophylls in dolsan leaf mustard (Brassia Juncea) Cho. Y.S.;Ha. B.S.;Park. S.K.;Chun. S.S.
  8. Ph.D. thesis Effect of kimchi inhibition of skin aging of hairless mouse Ryu. B.M.
  9. Ph.D. thesis Studies on scavenging activity of peroxynitrite and reactive oxygen species of green tea polyphenols Kye. I.S.
  10. M.S. thesis Oxidative stability of carrot carotenoids in the model systems. Song. E.S.
  11. J. Korean. soc. Food Sci. Nutr v.26 Chemical components in different parts of pumpkin Park. Y.K.;Cha. H.S.;Park. M.W.;Kang. Y.H.;Seog. H.M.
  12. Method and adaptation of sensory test Kim. K.O;Kim. s.S.;sung. P.K.;Lee. Y.C
  13. M.S. thesis Preparation methods and fermentation characteristics of leaf mustard kimchi Hyun. Y.A.
  14. J. Food Sci. v.28 Determination of chlorophylls, chlorophyllides, pheophytins and pheophorbides in plant material White. R.C.;Jones. I.D.;Eleanor. G.
  15. Association of official analytical chemists (15th ed) v.942 Official methods of analysis AOAC
  16. J. Food Science v.43 Phenolic antioxidants of dried soybeans Hammerschmidt. P.A.;Pratt. D.E.
  17. Theory and investigation of food analysis Shin. H.S
  18. J. Food Sci. v.44 Source of antioxidant activity of soybeans and soy products Partt. D.E.;Birac. P.M.
  19. J. Agric. Food Chem. v.40 Antioxidative substances in leaves of polygonum hydropiper Haraguchi. H.;Hashimoto. K.;Yagi. A.
  20. JAOCS v.66 Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media Farag. Rj.S;Badei. A.Z.M.A.
  21. Methods in Ezymol v.52 Mircosomal lipid peroxidation Bugge. J.A.;Aust. S.D.
  22. Methods in Enzymology v.105 Esterbauer. H.;Lang. J.;Zadravec. S.;Slater.T.F.
  23. Nature v.25 Antioxidant determinations by th use of a stable free radical Blois. M.S.
  24. User's Guide : Statistics SAS
  25. Korea Food Culture Research Changes in major taste components and microflora in mustard leaf kimchi during fermentation Cho. Y.s.;Park. S.G.
  26. J. Korean soc. Food Sci. Nutr. v.30 Fermentation characteristics of mustard leaf kimchi added green tea and pumpkin powder Park. M.J.;Jeon. Y.S.;Han. J.S.
  27. Ph D. thesis Antioxidative characteristics of chlorophylls and carotenoids in mustard leaf kimchi Song. E.S.
  28. JAOCS v.62 Antioxidant effects of chlorophyll and pheophytin on the antioxidation of oils in the dark.I. Comparision of the ingibition effects Enod. Y.;Usuki. R;Kaneda. T.
  29. PhD. thesis Studies on the antioxidative characteristics and antioxidative substance of kimchi Lee. Y.O.
  30. An AVI Book Pigments in vegetables. chlorophyll and carotenoids Gross. J.
  31. J. Korean Soc. food Sci. Nutr. v.29 Fermentation characteristics and antioxidative effect of red mustard leaf kimchi Hwang. J.H.
  32. Korean. J. Nutr. v.18 Effects of sugars on kimchi fermentation and on the stability of ascorbic acid. Jung. H.S.;Ko. Y.T.;Lim. S.J.
  33. J. Korean Soc. Food Sco. Nutr v.26 Chemical components in different parts of pumpkin Park. Y.K.;Cha. H.S.;Park. M.W.;Kang. Y.H.;Seog. H.M.
  34. M.S. thesis Antioxidative characteristics of red mustard leaf kimchi and crude anthocyanin pigment Hwang. J.H.