Selection of Lactic Acid Bacteria Specifically Inhibiting the Growth of Helicobacter pylori

Helicobacter pylori의 생육을 특이적으로 억제하는 유산균 선발

  • 정후길 (매일유업(주) 중앙연구소) ;
  • 김응률 (매일유업(주) 중앙연구소) ;
  • 전석락 (매일유업(주) 중앙연구소)
  • Published : 2001.06.01

Abstract

This study was conducted to select lactic acid bacteria which possess potential inhibitory effect on Helicobacter pylori, and to make feasibility test of fermented milk products using them. In order to select lactic acid bacteria specifically inhibiting the growth of H. pylori, antibacterial activity using paper disk method, adherence ability to Caco-2 cell inhibitory effect on urease activity of H. pylori, and milk fermentation feasibility were measured. Among 45 strains of lactic acid bacteria tested, 28 strains showed clear zone and Lactobacillus gasseri MK-03 showed the largest clear zone. Caco-2 cell adherence by lactic acid bacteria and inhibitory effect of them on H. pylori adherence were also evaluated. Of 28 strains tested, 18 strains appeared to be effective on adherence to Caco-2 cell, and especially Bifidobacterium longum MK-26 was found to be superior to others. When Bif. longum MK-26 and H. pylori were reacted with Caco-2 cell 2hrs before, adherence percentage of H. pylori decreased from 0.105% to 0.004%. To investigate inhibitory effect of lactic acid bacteria-derived supernatant on urease activity of H. pylori, pH-adjusted fermented supernatant(pH-4.4) was assessed by co-cultivation method. There of Lb. acidophilus MK-07-derived supernatant showed the most inhibitory effect on urease activity of H. pylori. Considering milk fermentation ability of selected 3 strains, they were comparably feasible to fermented milk products. Consequently, Lb. gasseri MK-03, Lb. acidophilus MK-07, and Bif. longum MK-26 were selected to specifically inhibit the growth of H. pylori, by antibacterial activity, inhibition of urease activity, and inhibition of Caco-2 cell adherence, respectively.

위염 및 위궤양, 심이지장궤양, 위암의 주요한 발병 원인균으로 알려져 있는 Helicobacter pylori의 생육을 특이적으로 억제하는 유산균을 선발하기 위해서, 45종의 유산균에 대하여 paper disk법을 이용한 항균 효과, Caco-2세포에 대한 정착능, urease activity법을 이용한 H. pylori 억제능 등을 파악하고, 유산균 배양액과 H. pylori의 동시배양에 따른 요소 분해효소의 활성 변화와 우유 발효능 등을 실험하였다. H. pylori의 생육을 억제하는 물질의 생성 유무를 확인하기 위하여 paper disk법으로 유산균 배양액에 의한 H. pylori의 생육 억제환을 측정한 결과, 28종의 유산균에서 생육 억제환이 형성되었다. 대부분이 유산 생성에 의한 낮은 pH 때문인 것으로 판단되지만, 일부 균종에서는 항균성 물질에 의한 억제환이 형성되었다. 다른 유산균에 비해서 생육 억제환이 가장 큰 Lactobacillus gasseri MK-03 균주가 H. pylori에 대한 항균 활성이 가장 우수한 것으로 나타났다. 항균 활성을 나타낸 28종의 유산균을 대항으로 Caco-2 세포에 대한 정착능을 실험한 결과, 18종의 유산균에서 정착능이 확인되었으며, Bifidobacterium longum MK-26 균주가 가장 우수한 것으로 나타났다. 특히 반응 2시간 전에 미리 Caco-2 세포에 정착시켰을 때, H. pylori의 정착율은 0.105%에서 0.004%로 감소되었다. 한편 요소 분해효소 활성을 저해하여 H. pylori를 억제하는 유산균을 선발하기 위해서 항균활성이 인정되는 28종의 유산균을 대상으로 실험한 결과, 21종의 유산균에서 요소 분해효소 활성의 저해 효과가 나타났으며, 이중에서 Lb. acidophilus MK-07 균주가 가장 우수한 것으로 판명되었다. 따라서 Lb. gasseri MK-03 균주는 항균 활성에서, Lb. acidophilus MK-07 균주는 요소 분해효소의 활성 억제에 의해서, Bif. longum MK-26 균주는 정착능 저해에 있어서 각각 우수한 H. pylori 생육 억제능을 나타냈다. 한편 최종적으로 선발된 3종의 유산균과 대조균주로서 13종의 유산균에 대해서는 우유 배양실험을 실시한 결과, 3종의 선발균주 모두가 발효유 제품에의 응용 적합성을 나타냈다.

Keywords

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