A study on the effect of side-lobe on axial image of confocal microscope

공초점 광학현미경에서의 side-lobe 감소방안에 대한 연구

  • 김억봉 (제주대학교 물리학과, 기초과학연구소) ;
  • 류재연 (제주대학교 물리학과, 기초과학연구소) ;
  • 최치규 (제주대학교 물리학과, 기초과학연구소) ;
  • 김두철 (제주대학교 물리학과, 기초과학연구소) ;
  • 유영훈 (제주대학교 물리학과, 기초과학연구소)
  • Published : 2001.10.01

Abstract

The effect of side-lobe on axial image is investigated. We show, using computer simulation and experimentally, that an optimized axial response can be achieved by minimized side-lobe according to the Herschel condition and that the appearance of sidelobes is decreased by balancing phase error.

공초점 현미경에서 광축 방향의 상을 얻을 때 잡음으로 작용하는 side-lobe의 영향을 조사하였다. 이러한 side-lobe는 Herschel 조건을 만족시킴으로써 최소화시킬 수 있다. 본 연구에서는 side-lobe에 대한 영향을 전산 시늉하였고, 이 결과를 실험에 적용하여 Herschel 조건에 의해 위상 보정을 함으로써 side-lobe가 최소화됨을 확인하였다.

Keywords

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