벤젠과 톨루엔 분해에 적합한 미소환경과 토착미생물군의 분포변화

Microbial Community in Various Conditions of Soil Microcosm

  • 이한웅 (고려대학교 생명공학원) ;
  • 이상현 (고려대학교 생명공학원) ;
  • 이정옥 (고려대학교 생명공학원) ;
  • 김현국 (고려대학교 생명공학원) ;
  • 이수연 (고려대학교 생명공학원) ;
  • 방성호 (한서대학교 생물학과) ;
  • 백두성 (고려대학교 지구환경학과) ;
  • 김동주 (고려대학교 지구환경학과) ;
  • 박용근 (고려대학교 생명공학원)
  • 발행 : 2001.03.01

초록

생물학적 방법으로 토착 미생물에 의해 벤젠과 톨루엔을 효과적으로 분해할 수 있는 토양환경인자의 조건을 조사하기 위해 16가지의 서로 다른 환경의 미소환경(microcosm)을 제작하여 벤젠과 톨루엔 분해실험을 수행하였고, 아울러 분해과정에서 토착미생물의 분포변화를 조사하였다. 그 결과 실험 조건중 토양의 수분 포화도는 30%와 60%이면서 동시에 생물들이 흡착할 수 있는 미생물 흡착제로 활성탄을 1% 첨가한 미소환경(Case 6, Case 7)에서 벤젠과 톨루엔의 분해속도가 가장 빨랐다. 토착토양미생물의 분포변화를 조사한 결과 벤젠과 톨루엔의 분해가 가장 빨리 일어나는 Case 6 와 Case 7에서는 10일 배양 후 total culturable bacteria는 초기 세균 수에 비해 각각 488배와 308배가 증가하였다. 벤젠과 톨루엔 분해세균의 증가 역시 총 세균수가 증가하는 비율로 증가하여 초기 분포를 계속 유지하였고, 벤젠과 톨루엔을 첨가한 미소환경에서 분해 미생물 종의 변화는, 첨가 전 그람음성 세균이 반응 10일 후에는 그람양성 세균이 탈이 분리되었다.

Biological treatment of benzene and toluene contaminated soil was investigated in laboratory microcosm of 16 different types for degrading benzene and toluene by indigenous bacteria. At the experimental conditions of the microcosms fast degrading benzene and toluene, moisture contents were 30% and 60% in a soil gap and content of powdered-activated carbon(PCA) for adhesion of benzene and toluene-degrading bacteria was 1% in total soil mass. At the conclusion of the shifted bacteria community, Case 6 and case 7 were operated until 10 days, and then the total cell number and the number of benzene and toluene degrading bacteria were investigated. The total cell number of Case 6 and Case 7 increased 488 fold and 308 fold of total indigenous cell, respectively. The number of benzene and toluene degrading bacteria increased and maintained the percentages occupied in pre-operating microcosm. Species of benzene and toluene degrading bacteria in microcosm changed from species of Gram negative bacteria to Gram positive bacterial species after soil exposed to benzene and toluene.

키워드

참고문헌

  1. Introduction to soil microbiology.(second ed.) Alexander, M.
  2. Appl. Envir. Microbiol. v.64 Genetic diversity of nifH gene sequences in Paenibacillus azotofixans strains and soil samples analyzed by denaturing gradient gel electrophoresis of PCR-amplified gene fragments Alexandre S.R.;G.F. Duarte;L. Seldin;J.D.V. Elsas
  3. Mutat. Res. v.145 Recent findings on the genetic toxicity of benzene, toluene, xylenes, and phenol Dean, B.J.
  4. Biocatalysis v.2 Initial studies on the regulation of toluene degradation by Pseudomonas putida F1 Finette, B.A.;D.T. Gibson
  5. Appl. Environ. Microbiol. v.57 Performance characterization of a model bioreactor for the biodegradation of trichoroethy1-ene by Pseudomonas cepacia G4 Folsom, B.R.;P.J. Chapman
  6. Appl. Environ. Microbiol. v.58 Isolation and characterization of a nitrite-reduction gene and its use as a probe for denitrifying bacteria Geoffrey, B.;Smith;J.M. Tiedje
  7. Manual of methods for general bacteriology Gerhart, P.;R.G.E. Murray;R. Cotillow;E. Nester;E.A. Wood;N. R. Krieg;G.B. Phillps
  8. Bergery's manual of determinative bacteriology(9th ed.) Holt, J.G.;Krieg N.R.;Sneath P.H.A.;Stalely J.T.;Williams S.T.
  9. Appl. Envir. Microbiol. v.65 Composition of toluene-degrading microbial communities from soil at different concentrations of toluene Hubert, C.;Y. Shen;G. Voordouw
  10. FEMS microbiology ecology v.33 Polyphasic microbial community analysis of petroleum hydrocarbon-contaminated soils from two northern Canadian communities Juck, D.;T. Charles;L.G. Whyte;C.W. Greer
  11. Bioprocess engineering v.23 Biodegradation of BTEX by bacteria on powdered activated carbon Mason, C.A.;G. Ward;K. Abu-Salah;O. Keren;C.G. Dosoretz
  12. FEMS Microbiology Ecology v.22 Production of auxin and other indolic and phenolic compounds by Paenibacillus polymyxa strains isolated from different proximity to plant roots Michael Lebuhn;T. Heulin;A. Hartmann
  13. Soil microbiology and biochemistry Paul, E.A.;F.F. Clark
  14. Petroleum contaminated soils v.2 Fingerprinting petroleum products: unleaded gasolines Potter, T.L.;P.T. Kostecki(ed.);E.J. Calabrese(ed.)
  15. Appl. Envir. Microbiol. v.66 Toluene-Degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene Parales, R.E.;J.L. Ditty;C.S. Harwood
  16. Chem. Eng. News v.70 Top 50 chemicals production stagnated last year Reisch, M.S.
  17. Appl. Envir. Microbiol. v.65 Microbial population changes during bioremediation of an experimental oil spill Sarah, J. MacNaughton;J.R. Stephen;A.D. Venosa;Gregory A. Davis;Yun-Juan Chang;David C. White
  18. Appl. Environ. Microbiol. v.65 Molecular analysis of microbial community structures in pristine and contaminated aquifers: field and laboratory microcosm experiments Shi, Y.;M.D. Zwolinski;M.E. Schreiber;J.M. Bahr;G.W. Sewell;W.J. Hickey
  19. Biodegradation v.1 The biodegradation of aromatic hydrocarbons by bacteria Smith, R.K.
  20. Appl. Envir. Microbiol. v.64 Metabolism of benzene, toluene, and xylene hydrocarbons in soil Tsao, C.-W.;H.-G. Song;R. Bartha