Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Comparative Study on Development of Mouse Embryos in Three Commercial Media and Hatching Rates of Mouse Embryos with/without Pronase

3개의 배양액내에서 생쥐배아의 발달과 Pronase로 처리한 생쥐배아 부화율의 비교 연구

  • Lee, Jeong-Heon (Department of Obstetrics and Gynecology, Chonbuk National University Hospital) ;
  • Go, Hee-Jeong (Department of Obstetrics and Gynecology, Chonbuk National University Hospital) ;
  • Chae, Geu-Jeong (Department of Obstetrics and Gynecology, Chonbuk National University Hospital) ;
  • Lee, Ki-Suk (Department of Obstetrics and Gynecology, Chonbuk National University Hospital) ;
  • Kim, Jong-Duk (Department of Obstetrics and Gynecology, Chonbuk National University Hospital)
  • 이정헌 (전북대학교 의과대학 산부인과학교실) ;
  • 고희정 (전북대학교 의과대학 산부인과학교실) ;
  • 채규정 (전북대학교 의과대학 산부인과학교실) ;
  • 이기숙 (전북대학교 의과대학 산부인과학교실) ;
  • 김종덕 (전북대학교 의과대학 산부인과학교실)
  • Published : 2001.09.30
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Abstract

Objectives: The purpose of this present study was to compare mouse embryo development in 3 commercial media and hatching competence of mouse embryo with or without enzymatic treatment. Methods: Collected 375 mouse embryos were divided into three groups, and then cultured in IVF-20 (G2), Medicult IVF (M3), P-1 (blastocyst M), respectively. Three day mouse morulae were cultured in G2 media treated with pronase. The results were analyzed using Chi-square test, and considered statistically significant when p<0.01. Results: The developmental rate of 2 cell mouse embryo after 72 hours was highest in IVF-20 (G2) among conventional 3 media. The hatching rate of mouse morulae was low when clultured in G2 media without pronase during 48 hours. However, it was higher when cultured in media treated with $1{\mu}g/ml$, $2.5{\mu}g/ml$, $5{\mu}g/ml$ pronase, respectively. Conclusions: Using good media and digestion of zona pellucida with enzymatic treatment improve development and hatching rate of embryo. Therefore, implantation and pregnancy rate could be improved.

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