Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Isolation and Identification of Acid- and Bile-Tolerant Lactobacillus salivarius subsp. salivarius from Human Faeces

  • Bae, Hyoung Churl (Division of Animal Science & Resources, College of Agriculture, Chungnam National University) ;
  • Choi, Seong Hyun (Department of Food Science & Technology, College of Agriculture, Chungnam National University) ;
  • Nam, Myoung Soo (Division of Animal Science & Resources, College of Agriculture, Chungnam National University)
  • Received : 2001.01.27
  • Accepted : 2001.03.19
  • Published : 2001.08.01
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Abstract

This study was conducted to isolate lactobacilli having characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from human faeces. Bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. Microhemagglutination tests using swine erythrocytes were performed to select lactobacilli having adherence properties to survive in the intestinal tract. By examination of these characteristics the strain Nam 27, which was isolated from adult faeces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing.

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Cited by

  1. Probiotic Characterization of Acid- and Bile-tolerant Lactobacillus salivarius subsp. salivarius from Korean Faeces vol.15, pp.12, 2002, https://doi.org/10.5713/ajas.2002.1798
  2. Purification and Characterization of α-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27 vol.27, pp.1, 2001, https://doi.org/10.5851/kosfa.2007.27.1.102
  3. Properties of β-Galactosidase from Lactobacillus salivarius subsp. salivarius Nam27 vol.27, pp.1, 2001, https://doi.org/10.5851/kosfa.2007.27.1.110