Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Establishment of diagnositc method for bovine neosporosis by PCR using paraffin block

파라핀 블록 PCR을 이용한 소 네오스포라 감염증의 진단법 확립

  • Lee, Jung-keun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jae-Hoon (National Veterinary Research Institute and Quarantine Service) ;
  • Kim, Jin-Hyun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Byung-chun (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Hwang, Woo-suk (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Youn, Hee-jeong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
  • Nam, Hoo-woo (Department of Parasitology, College of Medicine, Catholic University) ;
  • Jean, Young-wha (National Veterinary Research Institute and Quarantine Service) ;
  • Kim, Dae-Yong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
  • 이중근 (서울대학교 수의과대학, 농생명공학부) ;
  • 김재훈 (국립수의과학검역원) ;
  • 김진현 (서울대학교 수의과대학, 농생명공학부) ;
  • 이병천 (서울대학교 수의과대학, 농생명공학부) ;
  • 황우석 (서울대학교 수의과대학, 농생명공학부) ;
  • 윤희정 (서울대학교 수의과대학, 농생명공학부) ;
  • 남호우 (카톨릭의과대학 기생충학교실) ;
  • 진영화 (국립수의과학검역원) ;
  • 김대용 (서울대학교 수의과대학, 농생명공학부)
  • Accepted : 2001.08.23
  • Published : 2001.09.25
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Abstract

Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.

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