Korean Journal of Veterinary Research (대한수의학회지)
- Volume 41 Issue 2
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- Pages.177-188
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- 2001
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Immunogenicity of staphylococcal enterotoxin C mutant antigen in mice and dairy cows
포도상구균 장내 C 형 변이독소 (SEC mutant)의 면역원성에 대한 연구
- Chang, Byoung-sun (LG Chemical LTD.) ;
- Joo, Yi-seok (National Veterinary Research and Quarantine Service) ;
- Moon, Jin-san (National Veterinary Research and Quarantine Service) ;
- Seo, Keun-seok (Department of Microbiology, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University) ;
- Yang, Soo-jin (Department of Microbiology, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University) ;
- Kim, So-hyun (Department of Microbiology, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University) ;
- Park, Yong-ho (Department of Microbiology, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University)
- 장병선 (LG 화학) ;
- 주이석 (국립수의과학검역원) ;
- 문진산 (국립수의과학검역원) ;
- 서근석 (서울대학교 수의과대학 미생물학 교실 및 농생명공학부) ;
- 양수진 (서울대학교 수의과대학 미생물학 교실 및 농생명공학부) ;
- 김소현 (서울대학교 수의과대학 미생물학 교실 및 농생명공학부) ;
- 박용호 (서울대학교 수의과대학 미생물학 교실 및 농생명공학부)
- Accepted : 2001.04.16
- Published : 2001.06.25
Abstract
Mastitis is one of the most significant cause of economic loss to the dairy industry. Especially, Staphylococcus aureus is a major contagious mastitis-causing pathogen in dairy cattle. Because of its high transmission rate and resistance to antibiotic therapy, staphylococcal mastitis presents a constant threat to the dairy industry. Staphylococcal enterotoxin C(SEC) produced by S aureus has been known as one of superantigens which are able to stimulate a large proportion of T lymphocytes independently of their antigenic specificity. In this experiment, we have conducted preliminary studies with mice and lactating cows to evaluate the immunogenicity and safety of the experimental vaccine consists of SEC mutant antigen on controlling the bovine mastitis associated with S aureus infections. The average value of somatic cell counts in quarter milk, isolation rate of S aureus were consistently decreased in SEC-SER vaccinated groups, whereas antibody titers were highly increased in SEC-SER vaccinated groups. Peripheral blood were also collected from the lactating cows to determine the proportion of leukocyte subpopulation associated with humoral immunity(HI) and cell mediated immunity(CMI). Proportion of leukocyte subpopulation expressing