Korean Journal of Veterinary Research (대한수의학회지)
- Volume 41 Issue 4
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- Pages.535-542
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- 2001
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Multiplex PCR for differential diagnosis of Mycobacterium species from bovine clinical samples
소의 임상병리 가검물에서 Mycobacterium species 감별진단을 위한 multiplex PCR 기법
- Kim, Yong-hwan (Gwangju Metropolitan City Institute of Health and Environment) ;
- Al-Haddawi, MH (College of Veterinary Medicine, Chonnam National University) ;
- Cho, Ho-seong (College of Veterinary Medicine, Chonnam National University) ;
- Kang, Sung-kwi (College of Veterinary Medicine, Chonnam National University) ;
- Cho, Kyoung-oh (College of Veterinary Medicine, Chonnam National University) ;
- Park, Hyung-seon (College of Veterinary Medicine, Chonnam National University) ;
- Lee, Bong-joo (College of Veterinary Medicine, Chonnam National University) ;
- Park, Nam-yong (College of Veterinary Medicine, Chonnam National University)
- 김용환 (광주광역시보건환경연구원) ;
- ;
- 조호성 (전남대학교 수의과대학) ;
- 강성귀 (전남대학교 수의과대학) ;
- 조경오 (전남대학교 수의과대학) ;
- 박형선 (전남대학교 수의과대학) ;
- 이봉주 (전남대학교 수의과대학) ;
- 박남용 (전남대학교 수의과대학)
- Accepted : 2001.10.25
- Published : 2001.12.24
Abstract
A multiplex PCR technique was developed for detecting specifically each Mycobacterium bovis, M. tuberculosis, M. avium and M. avium subsp, paratuberculosis, respectively, using clinical samples of field cattle. To apply this novel technique to clinical specimens, blood sample was obtained from live cows comprising 11 intradermal tuberculin test (ITT)-positive and 17 ITT-negative and tested by multiplex PCR. Positive results were obtained from 15 cows by the multiplex PCR, showing that 4 (23.5%) of the 17 ITT-negative cows were multiplex PCR positive. The multiplex PCR results also showed that among the 15 positive cows, 7 (46.7%) were infected with M. bovis, 1 (6.7%) with M. tuberculosis and 7 (46.7%) with M. avium. The sensitivity and specificity of multiplex PCR in comparison with those of ITT were 100% and 76.5%. The correlation between the multiplex PCR and ITT assays with blood samples was considered excellent, 85.7% agreement and