Study on the Anti-cancer, Anti-metastasis and Effects of Immune-response of Aqua-acupuncture with Cuscutae Semen Infusion Solution

토사자약침(兎絲子藥鍼)의 항암작용(抗癌作用) 및 면역효과(免疫效果)에 대(對)한 실험적(實驗的) 연구(硏究)

Objective : The objective of tihs study is to study the effects of anti-cancer, anti-metastasis and effects of immune-response of aqua-acupuncture with Cuscutae Semen infusion solution. Methods : We observed, in-vitro, the cytotoxicity and the effect on the expression of MMP-9 gene, and in-vivo, change of body weight, surviving number, MST, ILS, changes in amount of WBC, RBC, PLT, GOT, GPT, creatinine, glucose and LDH, number of Pulmonary colony. Results : 1. The effect on expression of MMP-9 gene was decreased in all the sample groups in B16-F10 cell line, and was decreased in Lane 1, 2, 3 in HT1080 cell, compared with control group. 2. BALB/c mice which was transpianted S-180 cancer cell line were inhibited significantly in weight increase, in all the sample groups, compared with control group. 3. The sample groups injected in vein with B16-F10 cancer cell line in C57BL/6 mice did'nt show significant change in the number of WBC, RBC, PLT. 4. In immune experiment, all the sample groups showed having more relevancy to the effect on splenic cell proliferation than normal groups. 5. $IFN-{\gamma}$ and $TNF-{\alpha}$ in cytokine-gene were increased in all the sample groups than control group. 6. In flow cytometry of spienic cell, the numbers of CD4+ cell, CD8+ cell and CD19+ cell in sample groups were increased than in control group. Conclusion : Above the results showed that aqua-acupuncture of Cuscutae Semen infusion solution has effects of anti-cancer, anti-metastasis and immune response improvement.