BMB Reports
- Volume 34 Issue 4
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- Pages.299-304
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- 2001
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- 1976-670X(eISSN)
Regulation of 3-Deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) Synthase of Bacillus sp. B-6 Producing Phenazine-1-carboxylic acid
- Kim, Kyoung-Ja (Department of Life Science, Soonchunhyang University)
- Received : 2001.03.12
- Accepted : 2001.04.12
- Published : 2001.07.31
Abstract
The 3-Deoxy-D-arabinoheptulosonate 7-phosphate (DAHP) synthase is the first enzyme of aromatic amino acid-, folic acid-, and phenazine-1-carboxylic acid biosynthetic pathways. DAHP synthase of Bacillus sp. B-6 that produces phenazine-1-carboxylic acid was feedback inhibited by two intermediary metabolites of aromatic amino acid biosynthetic pathways, prephenate and chorismate, but not by other metabolites, such as anthranilic acid, shikimic acid, p-aminobenzoic acid, and 3-hydroxyanthranilic acid. DAHP synthase of Bacillus sp. B-6 was not inhibited by end products, such as aromatic amino acids, folic acid, and phenazine-1-carboxylic acid. The inhibition of DAHP synthase by prephenate and chorismate was non-competitive with respect to erythrose 4-phosphate and phosphoenolpyruvate. Prephenate and chorismate inhibited 50% of the DAHP synthase activity at concentrations of
Keywords
- 3-Deoxy-D-arabinoheptulosonate 7-phosphate (DAHP) synthase;
- Phenazine-1-carboxylic acid biosynthetic pathway;
- Aromatic amino acids;
- Prephenate;
- Chorismate