BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Osteoclast Differentiation Factor Engages the PI 3-kinase, p38, and ERK pathways for Avian Osteoclast Differentiation

  • Kim, Hong-Hee (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry, Chosun University) ;
  • Kim, Hyun-Man (Department of Oral Anatomy, College of Dentistry, Seoul National University) ;
  • Kwack, Kyu-Bum (Immunomodulation Research Center, University of Ulsan) ;
  • Kim, Si-Wouk (Research Center for Proteineous Materials, Chosun University) ;
  • Lee, Zang-Hee (National Research Laboratory for Bone Metabolism, Research Center for Proteineous Materials, and School of Dentistry, Chosun University)
  • Received : 2001.05.31
  • Accepted : 2001.06.25
  • Published : 2001.09.30
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Abstract

Osteoclasts, cells primarily involved in bone resorption, originate from the hematopoietic precursor cells of the monocyte/macrophage lineage and differentiate into multinucleated mature forms. We developed an in vitro osteoclast culture system using embryonic chicken bone marrow cells. This culture system can be utilized in studies on the differentiation and function of osteoclasts. Phosphatidylinositol 3-kinase (PI3-kinase) and mitogen-activated protein kinases (MAPKs) have been implicated in diverse cellular functions including proliferation, migration, and survival. Using the developed avian osteoclast culture system, we examined the involvement of these kinases in osteoclast differentiation by employing specific inhibitors of the kinases. We Found that the inhibition of the PI 3-kinase, p38, or ERK interfered with osteoclast formation, suggesting that the signaling pathways that involve these molecules participate in the process of chicken osteoclast differentiation.

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